在生物分析领域，准确检测复杂生物样本中的成分一直是个挑战。生物基质的干扰以及一些成分的半衰期短，都给分析工作带来了困难。为解决这些问题，科学家们想出了新办法。这次的研究结合了辅助示踪剂策略和便携式蒸汽渗透气体膜分离装置（PSPGMS）来检测过氧亚硝酸盐（ONOO⁻）。过氧亚硝酸盐是生物体内一种重要的活性物质，对它的准确检测有助于我们了解生物体内的生理和病理过程。研究中选用甲醇作为辅助示踪剂，因为它背景信号低且环保。在紫外线照射下，甲醇可以捕获活细胞中的游离过氧亚硝酸盐。然后利用PSPGMS装置进行分离、吸收和衍生化等操作，检测甲醇与过氧亚硝酸盐光解产物氧化反应生成的甲醛。这种方法效果显著，在2.0 - 20.0 μmol/L的浓度范围内有良好的线性响应，对大鼠肝细胞分析的回收率在87.4 - 115.0%之间，检测限为0.5 μmol/L。而且该方法具有出色的便携性和集成性，不需要大型昂贵的仪器，适合现场快速可视化检测，为生物分析提供了一种新的有效途径。

期刊

Analytical Chemistry

标题

Adjuvant Tracer Strategy Combined with a Portable Steam Penetration Gas Membrane Separation Device for Peroxynitrite Detection

作者

Chenbing Xi，Minyi Luo，Yong Zhang，Zhengyi Chen

摘要

Sample pretreatment is an indispensable part of the analysis of complex biological matrices to ensure the accuracy and reliability of analytical results, but it is rather difficult to develop a miniaturized and portable strategy to minimize matrix interference. In light of the difficulties resulted by interference from biological matrices and the short half-life of some constituents, this work introduces a combination of an adjuvant tracer with a high-efficiency and portable sample pretreatment device, the portable steam penetration gas membrane separation (PSPGMS) device, achieving analysis of complex biological samples under the interference of matrices. In this work, methanol was utilized as adjuvant to capture free ONOO– in living cells under UV irradiation for its low background signal and eco-friendliness; the PSPGMS device conducted all-in-one separation, absorption, and derivatization for the detection of formaldehyde generated by the oxidization between methanol and the products of ONOO– photolysis, achieving a linear response (R2 = 0.9919) across 2.0–20.0 μmol/L and a recovery rate of 87.4–115.0% in the analysis of rat hepatocytes. With a detection limit of 0.5 μmol/L, excellent portability, and integration, this strategy is well-suited for on-site rapid and visualized detection, independent of bulky and expensive instruments.

原文链接

https://pubs.acs.org/doi/10.1021/acs.analchem.5c04503

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