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冷泉港single cell analysis单细胞分析研讨会的相关内幕精华
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1. Jim Eberwine (Penn) reported some immature mRNA gets targeted to synapses by 'zip codes' in introns. splicing machinery exists in the synapse, the intron(s) get spliced, and protein made. Published work includes PubMed 21078998 and 21382548.
1. I already mentioned the OGT single cell array.
1. Lindsey M Costantini (works with Erik Snapp) had an excellent poster, "Single cell imaging approach to understanding homeostasis of the endoplasmic reticulum
environment: improving fluorescent proteins for the secretory pathway". For related work from same lab, see PubMed 21255213 (also various Snapp fluorescent protein reviews such as 19819147). ... note: I am a GFP aficionado, re: my thousand proteins of light PDF and Excel file at http://www.formatex.org/microscopy3/papers.htm ... if you prefer single cell transcriptomes, genomes and metabolomes, you should have gone to the meeting.
1. Takeharu Nagai, "Auto-luminescent imaging tools for combining use with optogenetic technology", spoke about "SuperStar" (improved Venus YFP-RLuc-8-X fusion protein) and "SuperBRAC", and a little about Nano-Cameleon. I will just mention that for anyone who has imaged firefly luciferase expressing cells in anesthetized mice on a Xenogen IVIS, that watching a video of a mouse recorded at 30 fps while wandering around in its cage is quite a change. I mentioned to Prof. Nagai that he should connect with Brian Rabinovich (MD Anderson Cancer Center - see Brian's PNAS paper, suppl. table) to improve RLuc (and maybe Venus) mRNA further.
1. I enjoyed the techno details and outcome of the talk by Sten Linnarsson, "Counting every mRNA molecule in single cells reveals allometric scaling of the
transcriptome". He mentioned the paper was coming out in Genome Research - see PubMed 21543516.
1. John D. McKinney, EPFL, had a terrific talk on mycobacteria (tuberculosis) single cell resistance mechanisms, "Individuality of bacterial responses to antibiotics". I did not see any single cell papers from him, so you will just have to keep an eye out for details as they get published.
1. I was familiar with most of the work by Takeaki Ozawa, "Real-time analysis of intracellular molecules and signaling using the protein fragment reconstitution techniques", but great to finally meet him (and Dr. Nagai, and Sunney Xie and Xiaowei Zhuang).
1. I may be a bit biased in this (have one, pssibility two nanoscope instrumentation grant proposals to submit in September, and hosting a Leica CW-STED demonstration late August-early Sept to get more data for the proposals), but:
Fluorescence nanoscopes are hot!
Stop buying confocal microscopes (unless they are the chassis for a nanoscope). Don't let your friend and neighbors and colleagues waste their money on just another confocal for cellular imaging. For latest example - Last week's latest fluorescence nanoscope paper - see fig S1 C vs D of http://www.pnas.org/content/suppl/2011/07/28/1107553108.DCSupplemental/pnas.201107553SI.pdf (for Fig 1 b vs c in main paper).
I am not claiming confocal and multiphoton fluorescence is dead - just that purchases should be limited to applications that are appropriate, such as http://www.pnas.org/content/108/31/12863.long
from my colleagues, which includes a nice method for efficient in vivo immunophenotyping。
CSHL 2011 Single Cell Analysis
workshop last week was outstanding. Big kudos to Jim Eberwine (Penn) and
Sunney Xie (Harvard), the co-organizers.
Jim ( http://www.linkedin.com/pub/james-eberwine/6/6bb/305
) is a co-inventor of a popular linear amplification method for RNA
(PubMed 8954839). Sunney just co-authored a Nature paper, Central dogma
at the single-molecule level in living cells. (PubMed 21776076).
Meeting summary is at
http://meetings.cshl.edu/meetings/single11.shtml
Xiaowei Zhuang gave an outstanding talk on fluorescence nanoscopes and
their use - particularly STORM and 3D-STORM invented by her and her lab.
Jim and Sunney announced that the next meeting will be at CSHL in March 2013, and then annually thereafter.
Group members here may want to keep an eye out for more from Oxford Gene
Technology on their 8100 single cells array for transcriptome capture
and analysis. The CSHL talk was much nicer than the current short video
at http://www.ogt.co.uk/singlecellvideo.html
I believe they understand nucleic acid hybridization pretty well - they were cofounded in 1990 by Edwin Southern.
++++++++++
哈哈，从以下名单知道了几个国内的同行。。。
Single Cell Analysis
July 22 - 24, 2011
Abstract Deadline: May 20, 2011
Organizers:
James
Eberwine, University of Pennsylvania Medical School
Xiaoliang Sunney Xie,
Harvard University
Typical
Program
We
are pleased to announce a second summer workshop on Single
Cell Techniques, which will be held at Cold Spring Harbor
Laboratory, New York. The meeting will begin with lunch and an
afternoon session at 2pm on Friday, July 22, and will conclude
in the late afternoon (5.30pm) on Sunday, July 24. The proposed
meeting will assemble the leaders in the field, together with
junior faculty, postdoctoral fellows and graduate students, to
discuss new, cutting-edge developments and techniques in single
cell analysis in bacterial, yeast, plant and animal systems.
Topics:
Genome Sequencing in
Single Cells
Transcriptome Analysis of Single Cells
Single Cell Proteomics
Single Cell Metabolomics
Quantitative Imaging in Single Cells
Modeling of Single Cell Biology
Keynote
Speaker
Xiaowei Zhuang, Harvard University
Confirmed
Speakers
Barry Cooperman, University pf Pennsylvania
Scott Fraser, California Institute of Technology
Jim Hicks, Cold Spring Harbor Laboratory
Junhyong Kim, University of Pennsylvania
Zaida Luthey-Schulten, University of Illinois
at Urbana-Champaign
Richard Novak, University of California, Berkeley
Hong Qian, University of Washington
Ben Simon, University of Cambridge
Jonathan Sweedler, University of Illinois
Fuchou Tang, Peking University
Jun Wang, Beijing Genomics Institute at Shenzhen
Barbara
Wold, California
Institute of Technology
The
format of the meeting will include six oral sessions, one poster
session and technical demonstrations. Each oral session will include
invited speakers and speakers selected from submitted abstracts.
For this reason, abstracts from accomplished junior and senior
investigators are warmly invited. As usual at Cold Spring Harbor
meetings, abstracts of both poster and platform sessions will
be published in an abstract book. The abstracts should focus on
new and unpublished data. The organizers will select abstracts
for oral or poster presentations. The status (talk/poster) of
abstracts will be posted on our web site (below) as soon as decisions
have been made. We have applied for funds from government and
industry to partially support graduate students and postdocs.
We hope to see you at Cold Spring Harbor in July.
This conference is supported in part by funds provided by the Cold Spring Harbor Corporate Sponsor Program and Molecular Devices
We have funds to provide partial scholarships for individuals who are US citizens/permanent residents from minority groups under-represented in the life sciences. Please provide justification in writing to meetings@cshl.edu and state your financial needs. Preference will be given to those applying who submit abstracts to the meeting.
Pricing
Academic Package $1055
Graduate/PhD Student Package $880
Corporate Package $1340
Academic/Student No-Housing Package $720
Corporate No-Housing Package $905
Currency converter
Regular packages are all inclusive and cover registration, food, housing, parking, wine-and-cheese party, lobster banquet, etc. No Housing packages include all costs except housing. Full payment is due 4 weeks prior to the meeting.
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