小柯机器人

四川农业大学Linkai Huang和武汉大学Shilin Tian共同合作，近期取得重要工作进展。他们通过泛基因组分析鉴定出了与珍珠粟耐热性相关的结构变异。相关研究成果2023年3月2日在线发表于《自然—遗传学》杂志上。

据介绍，珍珠粟是世界范围内重要的谷类作物，具有优异的耐热性。

研究人员通过将10个染色体基因组与一个适应全球不同气候的现有组装组合在一起，开发了一个基于图的泛基因组，并捕获了424085个基因组结构变异（SV）。比较基因组学和转录组学分析揭示了RWP-RK转录因子家族的扩增以及内质网（ER）相关基因在耐热性中的参与。一个RWP-RK基因的过表达导致植物耐热性增强，并快速反式激活ER相关基因，支持RWP-RK转录因子和ER系统在耐热性中的重要作用。

此外，研究人员发现一些SV影响了与耐热性相关的基因表达，而围绕ER相关基因的SV在驯化过程中形成了对耐热性的适应。

总之，这一研究提供了一个全面的基因组图谱资源，揭示了对耐热性的见解，并为在不断变化的气候下培育出更健壮的作物奠定了基础。

附：英文原文

Title: Pangenomic analysis identifies structural variation associated with heat tolerance in pearl millet

Author: Yan, Haidong, Sun, Min, Zhang, Zhongren, Jin, Yarong, Zhang, Ailing, Lin, Chuang, Wu, Bingchao, He, Min, Xu, Bin, Wang, Jing, Qin, Peng, Mendieta, John Pablo, Nie, Gang, Wang, Jianping, Jones, Chris S., Feng, Guangyan, Srivastava, Rakesh K., Zhang, Xinquan, Bombarely, Aureliano, Luo, Dan, Jin, Long, Peng, Yuanying, Wang, Xiaoshan, Ji, Yang, Tian, Shilin, Huang, Linkai

Issue&Volume: 2023-03-02

Abstract: Pearl millet is an important cereal crop worldwide and shows superior heat tolerance. Here, we developed a graph-based pan-genome by assembling ten chromosomal genomes with one existing assembly adapted to different climates worldwide and captured 424,085 genomic structural variations (SVs). Comparative genomics and transcriptomics analyses revealed the expansion of the RWP-RK transcription factor family and the involvement of endoplasmic reticulum (ER)-related genes in heat tolerance. The overexpression of one RWP-RK gene led to enhanced plant heat tolerance and transactivated ER-related genes quickly, supporting the important roles of RWP-RK transcription factors and ER system in heat tolerance. Furthermore, we found that some SVs affected the gene expression associated with heat tolerance and SVs surrounding ER-related genes shaped adaptation to heat tolerance during domestication in the population. Our study provides a comprehensive genomic resource revealing insights into heat tolerance and laying a foundation for generating more robust crops under the changing climate.

DOI: 10.1038/s41588-023-01302-4

Source: https://www.nature.com/articles/s41588-023-01302-4

Nature Genetics：《自然—遗传学》，创刊于1992年。隶属于施普林格·自然出版集团，最新IF：41.307
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