小柯机器人

上海交通大学Baojie Li和重庆陆军军医大学Lin Chen共同合作，近期取得重要工作进展。他们研究发现Prrx1标记成年小鼠的骨、白色脂肪组织和真皮的干细胞。相关研究成果2022年12月1日在线发表于《自然—遗传学》杂志上。

据介绍，特化的结缔组织，包括骨骼和脂肪组织，控制各种生理活动，包括矿物质和能量稳态。然而，在整个成年期维持这些组织的干细胞身份仍然不清楚。

通过在敲入Cre/CreER^T2细胞系中进行遗传谱系追踪和细胞耗竭实验，研究人员发现在成年小鼠中，罕见的Prrx1表达细胞充当骨骼、白色脂肪组织和真皮的干细胞，这些细胞对于这些组织的稳态和修复是不可或缺的。单细胞图谱分析揭示了Prrx1表达细胞的周期性和多能性质，移植试验进一步验证了这些细胞的干细胞性。

此外，研究人员还鉴定了表达Prrx1干细胞的细胞表面标记物，并表明这些干细胞活性受Wnt信号的调节。这些发现扩展了人们对结缔组织稳态和再生的了解，可能有助于改进基于干细胞的治疗方法。

附：英文原文

Title: Prrx1 marks stem cells for bone, white adipose tissue and dermis in adult mice

Author: Liu, Huijuan, Li, Ping, Zhang, Shaoyang, Xiang, Jinnan, Yang, Ruichen, Liu, Jiajia, Shafiquzzaman, Md, Biswas, Soma, Wei, Zhanying, Zhang, Zhenlin, Zhou, Xin, Yin, Feng, Xie, Yangli, Goff, Stephen P., Chen, Lin, Li, Baojie

Issue&Volume: 2022-12-01

Abstract: Specialized connective tissues, including bone and adipose tissues, control various physiological activities, including mineral and energy homeostasis. However, the identity of stem cells maintaining these tissues throughout adulthood remains elusive. By conducting genetic lineage tracing and cell depletion experiments in newly generated knock-in Cre/CreERT2 lines, we show here that rare Prrx1-expressing cells act as stem cells for bone, white adipose tissue and dermis in adult mice, which are indispensable for the homeostasis and repair of these tissues. Single-cell profiling reveals the cycling and multipotent nature of Prrx1-expressing cells and the stemness of these cells is further validated by transplantation assays. Moreover, we identify the cell surface markers for Prrx1-expressing stem cells and show that the activities of these stem cells are regulated by Wnt signaling. These findings expand our knowledge of connective tissue homeostasis/regeneration and may help improve stem-cell-based therapies.

DOI: 10.1038/s41588-022-01227-4

Source: https://www.nature.com/articles/s41588-022-01227-4

Nature Genetics：《自然—遗传学》，创刊于1992年。隶属于施普林格·自然出版集团，最新IF：41.307
官方网址：https://www.nature.com/ng/
投稿链接：https://mts-ng.nature.com/cgi-bin/main.plex