小柯机器人

研究揭示牛调控变异的多组织图谱
2022-08-12 15:59

丹麦奥尔胡斯大学Lingzhao Fang、英国爱丁堡大学Albert Tenesa和美国农业部动物基因组学和改良实验室George E. Liu研究组合作,揭示了牛调控变异的多组织图谱。这一研究成果于2022年8月11日发表在国际学术期刊《自然—遗传学》上。

研究人员基于7,180个已公开可用的RNA测序(RNA-seq)样本构建了一个牛基因型-组织表达图谱(CattleGTEx),这是研究社区农场动物GTEx(FarmGTEx)项目试点阶段的一部分。研究人员绘制了100多种组织/细胞类型的转录组学图谱,并揭示了数十万种与23种不同组织基因表达和可变剪接的遗传关联。研究评估这些遗传调控效应的组织共享模式,并使用多组学数据对其进行功能注释。

最后,使用转录组范围的关联和共定位分析将不同组织中的基因表达与43个在经济上具有重要性的性状联系起来,以破译牛这种农艺性状的分子调控机制。

据了解,揭示牲畜基因表达遗传调控变异对于解释具有经济价值性状的分子机制,以及通过人工选择提高遗传增益率至关重要。

附:英文原文

Title: A multi-tissue atlas of regulatory variants in cattle

Author: Liu, Shuli, Gao, Yahui, Canela-Xandri, Oriol, Wang, Sheng, Yu, Ying, Cai, Wentao, Li, Bingjie, Xiang, Ruidong, Chamberlain, Amanda J., Pairo-Castineira, Erola, DMellow, Kenton, Rawlik, Konrad, Xia, Charley, Yao, Yuelin, Navarro, Pau, Rocha, Dominique, Li, Xiujin, Yan, Ze, Li, Congjun, Rosen, Benjamin D., Van Tassell, Curtis P., Vanraden, Paul M., Zhang, Shengli, Ma, Li, Cole, John B., Liu, George E., Tenesa, Albert, Fang, Lingzhao

Issue&Volume: 2022-08-11

Abstract: Characterization of genetic regulatory variants acting on livestock gene expression is essential for interpreting the molecular mechanisms underlying traits of economic value and for increasing the rate of genetic gain through artificial selection. Here we build a Cattle Genotype–Tissue Expression atlas (CattleGTEx) as part of the pilot phase of the Farm animal GTEx (FarmGTEx) project for the research community based on 7,180 publicly available RNA-sequencing (RNA-seq) samples. We describe the transcriptomic landscape of more than 100 tissues/cell types and report hundreds of thousands of genetic associations with gene expression and alternative splicing for 23 distinct tissues. We evaluate the tissue-sharing patterns of these genetic regulatory effects, and functionally annotate them using multiomics data. Finally, we link gene expression in different tissues to 43 economically important traits using both transcriptome-wide association and colocalization analyses to decipher the molecular regulatory mechanisms underpinning such agronomic traits in cattle.

DOI: 10.1038/s41588-022-01153-5

Source: https://www.nature.com/articles/s41588-022-01153-5

Nature Genetics:《自然—遗传学》,创刊于1992年。隶属于施普林格·自然出版集团,最新IF:41.307
官方网址:https://www.nature.com/ng/
投稿链接:https://mts-ng.nature.com/cgi-bin/main.plex


本期文章:《自然—遗传学》:Online/在线发表

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