小柯机器人

荷兰阿姆斯特丹大学Leendert W. Hamoen、Gaurav Dugar等研究人员合作发现，一个染色体环锚介导细菌的基因组组织。2022年1月24日，《自然—遗传学》杂志在线发表了这项成果。

研究人员使用一种基于全基因组沉降的方法，发现一种转录因子Rok在枯草杆菌中形成大型核蛋白复合物。利用染色体构象捕捉和DNA位点的活体成像，研究人员表明这些复合物在很大的距离上有力地相互作用。重要的是，这些依赖Rok的长距离相互作用导致了固定的染色体环形成，从而在空间上隔离了大段的DNA，正如以前在真核生物中观察到的绝缘体蛋白。

据介绍，核蛋白复合物在真核生物和原核生物的基因组组织中都发挥着不可或缺的作用。除了在局部结构和压实DNA方面的作用外，一些复合物还通过介导长距离锚定染色体环的形成，导致大段DNA的空间分离来影响全局组织。在真核生物中，这种大范围的相互作用是无处不在的，但在原核生物中还没有被证实。

附：英文原文

Title: A chromosomal loop anchor mediates bacterial genome organization

Author: Dugar, Gaurav, Hofmann, Andreas, Heermann, Dieter W., Hamoen, Leendert W.

Issue&Volume: 2022-01-24

Abstract: Nucleoprotein complexes play an integral role in genome organization of both eukaryotes and prokaryotes. Apart from their role in locally structuring and compacting DNA, several complexes are known to influence global organization by mediating long-range anchored chromosomal loop formation leading to spatial segregation of large sections of DNA. Such megabase-range interactions are ubiquitous in eukaryotes, but have not been demonstrated in prokaryotes. Here, using a genome-wide sedimentation-based approach, we found that a transcription factor, Rok, forms large nucleoprotein complexes in the bacterium Bacillus subtilis. Using chromosome conformation capture and live-imaging of DNA loci, we show that these complexes robustly interact with each other over large distances. Importantly, these Rok-dependent long-range interactions lead to anchored chromosomal loop formation, thereby spatially isolating large sections of DNA, as previously observed for insulator proteins in eukaryotes. Hi-C and live-imaging data show that nucleoprotein complexes containing the transcription factor Rok interact over long distances in the bacterium Bacillus subtilis. Rok-dependent interactions contribute to anchored chromosomal loop formation.

DOI: 10.1038/s41588-021-00988-8

Source: https://www.nature.com/articles/s41588-021-00988-8

Nature Genetics：《自然—遗传学》，创刊于1992年。隶属于施普林格·自然出版集团，最新IF：41.307
官方网址：https://www.nature.com/ng/
投稿链接：https://mts-ng.nature.com/cgi-bin/main.plex