2020年9月28日出版的《自然-遗传学》杂志发表了以色列魏茨曼科学研究所Asaph Aharoni和Jdrzej Szymański研究团队研究。他们对基因渗入野生番茄的分析阐明了与果实性状和病原体反应相关转录组和代谢组变异的遗传基础。
研究人员利用表征野生荒漠适应性和驯化品系的基因渗入品种，来探索野生物种相关果实性状转移过程中基因表达和化学变异的遗传基础。利用580个品系的转录组和代谢组分析与病原体敏感性测定相结合的方法，研究人员鉴定出与数百种转录本和代谢产物水平相关的基因组位点。这些关联发生在代谢途径和成熟相关过程的协调扰动热点中。研究人员确定了茄类生物碱途径的组分，以及参与病原体防御并将真菌抗性与果实成熟调控网络变化联系起来的基因和代谢产物。
该研究结果揭示了番茄果实成熟过程中新陈代谢和病原体抗性的基础，并提供了对果实品质性状的见解。
据介绍，野生番茄代表了丰富的基因库，可用于探究驯化过程中许多失去的特性。
附：英文原文
Title: Analysis of wild tomato introgression lines elucidates the genetic basis of transcriptome and metabolome variation underlying fruit traits and pathogen response
Author: Jdrzej Szymaski, Samuel Bocobza, Sayantan Panda, Prashant Sonawane, Pablo D. Crdenas, Justin Lashbrooke, Avinash Kamble, Nir Shahaf, Sagit Meir, Arnaud Bovy, Jules Beekwilder, Yury Tikunov, Irene Romero de la Fuente, Dani Zamir, Ilana Rogachev, Asaph Aharoni
Issue&Volume: 2020-09-28
Abstract: Wild tomato species represent a rich gene pool for numerous desirable traits lost during domestication. Here, we exploited an introgression population representing wild desert-adapted species and a domesticated cultivar to establish the genetic basis of gene expression and chemical variation accompanying the transfer of wild-species-associated fruit traits. Transcriptome and metabolome analysis of 580 lines coupled to pathogen sensitivity assays resulted in the identification of genomic loci associated with levels of hundreds of transcripts and metabolites. These associations occurred in hotspots representing coordinated perturbation of metabolic pathways and ripening-related processes. Here, we identify components of the Solanum alkaloid pathway, as well as genes and metabolites involved in pathogen defense and linking fungal resistance with changes in the fruit ripening regulatory network. Our results outline a framework for understanding metabolism and pathogen resistance during tomato fruit ripening and provide insights into key fruit quality traits. A study of 580 tomato lines resulting from a cross between a wild desert-adapted species and a domesticated cultivar elucidates the genetic basis of gene expression and metabolite variation associated with fruit traits and defense against pathogens.
DOI: 10.1038/s41588-020-0690-6
Source: https://www.nature.com/articles/s41588-020-0690-6
Nature Genetics：《自然—遗传学》，创刊于1992年。隶属于施普林格·自然出版集团，最新IF：41.307
官方网址：https://www.nature.com/ng/
投稿链接：https://mts-ng.nature.com/cgi-bin/main.plex
本期文章：《自然—遗传学》：Online/在线发表
