今天和朋友交谈说道侧翼序列扩增的事,我说最近看到一篇扩增侧翼序列的新方法。就是这篇了Restriction Site Extension PCR: A Novel Method for High Throughput Characterization of Tagged DNA Fragments and Genome Walking High Throughput Characterization of Tagged DNA ...
T-DNA插入突变,是一种常用的大规模的产生突变体的方法。其基本原理是:T-DNA插入到功能基因,产生功能失活突变。但T-DNA的插入可能还会有其它效应,如这篇文章报道的:Chromosomal Translocations are a Common Phenomenon in Arabidopsis thaliana T-DNA Insertion Lines。 Chr ...
这篇也将是一篇不断更新的博文,关注QTL精细定位. 1 Fine mapping of Sta1 , a quantitative trait locus determining stele transversal area, on rice chromosome 9(得到全文再上传,有空再把一些旧资料补上) 2.Bai X, Luo L, Yan W, Kovi Mallikarjuna R, Zhan W, ...
Regulation of flowering in rice:twoflorigengenes,acomplex gene network,andnaturalvariation Regulation of flowering in rice 。这里面的遗传控制图应该是最新的,有新的水稻抽穗期基因出来可以添到图中,如果正在克隆,应该在里面找到相应的位置。 还有篇08年的综述Florigen and the Photoperiodic Control of ...
一:将QTL分解为单个孟德尔因子-----遗传背景简单化(单片段代换系) 二:利用大型或超大型分离群体获得足够多的统计学参数(NAM...) 例子Dissection of genetically complex traits with extremely large pools of yeast segregants Dissection of genetically ...