氢气治疗心肌缺血再灌注损伤目前已经有3篇论文，第一篇是来自日本医科大学太田的老年病研究所，是2008年发表的第一篇关于氢治疗疾病的论文，引用情况仅次于他们07年发表在 Nature Med的第一篇关于氢气治疗脑缺血的论文。是采用呼吸氢气（2%）证明对大鼠心肌缺血再灌注损伤具有治疗作用，该论文最著名的是关于各种气体信号的讨论，他们提出一氧化碳、一氧化氮和硫化氢虽然重要，可以保护细胞损伤，但毒性非常强。而氢气是“没有任何毒性的药物”。第二篇是我们实验室去年发表的论文，是采用腹腔注射氢气生理盐水治疗心肌缺血再灌注损伤，主要观察细胞凋亡，结合心脏功能和氧化损伤相关指标。本篇论文已经被International Journal of Cardiology接受。也将是国内氢气生物学研究领域论文发表杂志分数最高的（3.47）。（需要加油！）当然不得不提的是，关于心脏方面的研究，还有一篇来自美国匹大移植中心Nakao教授小组，关于氢气治疗心脏移植后损伤方面的。

本研究也是我们前篇论文的延续,仍是腹腔注射氢气生理盐水治疗心肌缺血再灌注损伤，不过重点观察与炎症相关的指标，例如ICAM-1、MPO、TNF-a和IL-1b等指标。研究结果表明，氢气治疗心肌缺血再灌注损伤主要与氢气可降低缺血再灌注心肌炎症反应有关。

该研究是在上海长海医院胸心外科王志农教授指导下完成的。我们是主要合作单位参与了部分工作。将来我们将继续深入该项研究，初步计划从自噬、重要免疫细胞（TH17和Treg）和内质网应激，结合重要氧化相关信号分子等方面。

 

ANTI-INFLAMMATORY EFFECT OF HYDROGEN-RICH SALINE IN A RAT MODEL OF REGIONAL MYOCARDIAL ISCHEMIA AND REPERFUSION

 

Yufeng ZHANG^a,1, Qiang SUN^b,1, Bin HE^a, Jian XIAO^a, Zhinong WANG^a*, Xuejun SUN^b*,

a Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P. R. China;

b Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, P. R. China;

¹ Yufeng ZHANG and Qiang SUN contributed equally to this work

* Corresponding author: Zhinong WANG and Xuejun SUN

Tel & Fax: +86-21-81873327.

E-mail address: zyf19810824@163.com

ABSTRACT

Introduction:  To study the possible anti-inflammatory effect of hydrogen-rich saline (H₂ saline) on rat hearts with regional myocardial ischemia and reperfusion (I/R).

Methods:  Sixty-six rats were equally randomized to three groups: sham-operated group, I/R group (control group) and I/R plus H₂ saline treatment group. Myocardial I/R was established by occlusion of the left anterior descending (LAD) coronary artery for 30 min and reperfusion for 24 h.

Results:  H₂ saline treatment attenuated I/R-induced cardiac cell apoptosis, presenting as significant improvement of heart function parameters 24 h after reperfusion, including left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVEDP), +(dP/dt)max and -(dP/dt)max. It also decreased neutrophil infiltration, 3-nitrotyrosine level, expression of intercellular adhesion molecule 1(ICAM-1) and myeloperoxidase (MPO) activity in the area at risk zones (AAR) of rat hearts subjected to regional myocardial I/R, and attenuated the increase of I/R induced pro-inflammatory cytokine tumor necrosis factor-α (TNF-a) and interleukin-1β (IL-1b) levels in the AAR.

Conclusion:  H₂ saline has an anti-inflammatory effect on rat hearts with regional myocardial I/R.

KEYWORDS

Myocardium, ischemia, reperfusion, inflammation, hydrogen-rich saline

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