SlWRKY35 positively regulates carotenoid biosynthesis by activating the MEP pathway in tomato fruit

第一作者：Yong Yuan

第一单位：四川大学

第一通讯：Yang Zhang

Abstarct

背景+意义：Carotenoids are vital phytonutrients widely recognized for their health benefits. Therefore, it is vital to thoroughly investigate the metabolic regulatory network underlying carotenoid biosynthesis and accumulation to open new leads towards improving their contents in vegetables and crops.

类胡萝卜素是重要的植物营养物质，被普遍认为对人类健康十分有益。因此，对于类胡萝卜素生物合成和积累的代谢调控网络研究，有助于我们进一步提升其在蔬菜和作物中的含量。

主要发现：The outcome of our study defines SlWRKY35 as a positive regulator of carotenoid biosynthesis in tomato.

本文中，作者发现在番茄中，SlWRKY35是类胡萝卜素生物合成的正向调控子。

结果1-SlWRKY35-SlDXS1-MEP-carotenoid：SlWRKY35 can directly activate the expression of the 1-deoxy-D-xylulose 5-phosphate synthase (SlDXS1) gene to reprogram metabolism towards the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, leading to enhanced carotenoid accumulation.

SlWRKY35能够直接激活1-脱氧-D-木酮糖-5-磷酸合酶编码基因SlDXS1的表达，从而重编程通向MEP途径的代谢，最终导致类胡萝卜素的积累增加。

结果2-SIRIN-SlWRKY35：We also show that the master regulator SlRIN directly regulates the expression of SlWRKY35 during tomato fruit ripening.

作者还发现，在番茄果实成熟过程中，主要调控子SlRIN能够直接调控SlWRKY35基因的表达。

结果3-SILCYE+SlWRKY35：Compared to the SlLCYE overexpression lines, coexpression of SlWRKY35 and SlLCYE can further enhance lutein production in transgenic tomato fruit, indicating that SlWRKY35 represents a potential target towards designing innovative metabolic engineering strategies for carotenoid derivatives.

与SlLCYE过表达株系相比，共表达SlWRKY35和SlLCYE能够进一步增强转基因番茄果实中叶黄素的产量，表明SlWRKY35是一个能够用于提升类胡萝卜素衍生物代谢工程的潜在靶标。

结论：In addition to providing new insights into the metabolic regulatory network associated with tomato fruit ripening, our data define a new tool for improving fruit content in specific carotenoid compounds.

本文的研究结果除了能够为番茄果实成熟相关代谢调控网络提供新的视野，另外也为提升果实中特定类胡萝卜素化合物的含量提供了新的工具。