The transcription factor MYB40 is a central regulator in arsenic resistance in Arabidopsis
第一作者:Yun Chen
第一单位:中国农业大学
第一通讯:Yi-Fang Chen
Abstract
背景回顾:Arsenic is a metalloid that is toxic to plants. Arsenate (As(V)), the prevalent chemical form of arsenic, is a phosphate (Pi) analog and is incorporated into plant cells via Pi transporters. 主要发现:Here, we found that the MYB40 transcription factor played important roles in the control of Arabidopsis As(V) resistance. 结果1-MYB40表达模式:The expression of MYB40was induced by As(V) stress. 结果2-MYB40过表达和突变表型:MYB40-overexpressing lines had an obvious As(V)-resistant phenotype and a reduced As(V)/Pi uptake rate, whereas myb40 mutants were sensitive to As(V) stress. 结果3-MYB40-PHT1;1:Upon exposure to As(V), MYB40 directly repressed the expression of PHT1;1, which encodes a main Pi transporter. The As(V)-resistant phenotypes of MYB40-overexpressing lines were impaired by overexpression of PHT1;1, demonstrating an epistatic genetic relationship between MYB40 and PHT1;1. 结果4-MYB40-PCS1和-ABCC1/2:Moreover, overexpression of MYB40 enhanced, and disruption of MYB40 reduced, thiol-peptide contents. Upon exposure to As(V), MYB40 positively regulated the expression of PCS1, which encodes a phytochelatin synthase, and ABCC1 and ABCC2, which encode the major vacuolar phytochelatin transporters. 结论:Together, our data demonstrate that AtMYB40 acts as a central regulator of As(V) responses, providing a genetic strategy for enhancing plant As(V) tolerance and reducing As(V) uptake to improve food safety. 摘 要
砷是一种对植物有毒的类金属。砷的主要化学形式是砷酸盐As(V),是一种磷酸盐(Pi)类似物,通过Pi转运蛋白被植物细胞吸入。本文中,作者发现MYB40转录因子在拟南芥As(V)抗性中发挥重要作用。MYB40基因的表达受到As(V)胁迫的诱导。MYB40过表达株系具有明显的As(V)抗性,植株对于As(V)/Pi的摄取率降低,而myb40突变株对As(V)胁迫较为敏感。在As(V)处理后,MYB40会直接抑制PHT1;1基因的表达,该基因编码一个主要的Pi转运体。在MYB40过表达株系中过表达PHT1;1基因,会导致原本高As(V)抗性表型减弱,说明MYB40与PHT1;1之间存在上位性遗传关系。此外,MYB40过表达增强了巯基肽的含量,而MYB40表达受损会降低巯基肽的含量。在As(V)处理后,MYB40会正向调控植物螯合肽合酶编码基因PCS1和主要液泡植物螯合肽转运蛋白编码基因ABCC1和ABCC2的表达。综上,本文的研究结果表明,AtMYB40作为As(V)响应的核心调控子,为提高植物的As(V)耐受性和减少As(V)吸收以改善食品安全提供了遗传策略。 通讯作者 ** 陈益芳 **
个人简介: 1992-1996年,湖南师范大学,学士; 1996-1998年,湖南常德市第七中学,生物教师; 1998-2003年,中国农业大学,博士; 2003-2008年,中国农业大学,讲师; 2009-2014年,中国农业大学,副教授; 2015年-至今,中国农业大学,教授。 研究方向:植物磷营养高效的生理及分子机制。
doi: https://doi.org/10.1016/j.xplc.2021.100234
Journal: Plant Communications
Published date: August 19, 2021
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