小柯机器人

美国洛克菲勒大学Gabriel D. Victora等研究人员合作实现体内免疫细胞相互作用的通用记录。该研究于2024年3月6日发表于国际一流学术期刊《自然》杂志上。

研究人员表示，免疫细胞依靠与其他免疫和非免疫群体的短暂物理相互作用来调节自身功能。为了在体内直接研究这些“亲完就跑”的相互作用，研究人员之前开发了LIPSTIC（通过SorTagging细胞间接触标记免疫伙伴关系），这种方法利用分子伴侣CD40L和CD40之间标记底物的酶转移来标记相互作用的细胞。然而，对这种途径的依赖限制了LIPSTIC在测量CD4⁺ T辅助细胞和抗原递呈细胞之间相互作用方面的应用。

研究人员报告了通用版LIPSTIC（uLIPSTIC）的开发，它可以记录免疫细胞之间以及免疫和非免疫群体之间的物理相互作用，而不受受体和配体的影响。研究表明，uLIPSTIC可用于监测树突状细胞对CD8⁺ T细胞的吸引，揭示调节性T细胞的稳态细胞伙伴，并根据其与生发中心B细胞的互动来识别生发中心驻留的T滤泡辅助细胞。通过将uLIPSTIC与单细胞转录组学相结合，研究人员建立了在稳定状态下与肠上皮细胞发生物理相互作用的免疫群体目录，并描绘了全身感染后多个器官中淋巴细胞色素膜炎病毒特异性CD8⁺ T细胞相互作用组的演变过程。因此，uLIPSTIC为测量和了解多个生物系统中的细胞-细胞相互作用提供了一种广泛有用的技术。

附：英文原文

Title: Universal recording of immune cell interactions in vivo

Author: Nakandakari-Higa, Sandra, Walker, Sarah, Canesso, Maria C. C., van der Heide, Verena, Chudnovskiy, Aleksey, Kim, Dong-Yoon, Jacobsen, Johanne T., Parsa, Roham, Bilanovic, Jana, Parigi, S. Martina, Fiedorczuk, Karol, Fuchs, Elaine, Bilate, Angelina M., Pasqual, Giulia, Mucida, Daniel, Kamphorst, Alice O., Pritykin, Yuri, Victora, Gabriel D.

Issue&Volume: 2024-03-06

Abstract: Immune cells rely on transient physical interactions with other immune and non-immune populations to regulate their function1. To study these ‘kiss-and-run’ interactions directly in vivo, we previously developed LIPSTIC (labelling immune partnerships by SorTagging intercellular contacts)2, an approach that uses enzymatic transfer of a labelled substrate between the molecular partners CD40L and CD40 to label interacting cells. Reliance on this pathway limited the use of LIPSTIC to measuring interactions between CD4+ T helper cells and antigen-presenting cells, however. Here we report the development of a universal version of LIPSTIC (uLIPSTIC), which can record physical interactions both among immune cells and between immune and non-immune populations irrespective of the receptors and ligands involved. We show that uLIPSTIC can be used, among other things, to monitor the priming of CD8+ T cells by dendritic cells, reveal the steady-state cellular partners of regulatory T cells and identify germinal centre-resident T follicular helper cells on the basis of their ability to interact cognately with germinal centre B cells. By coupling uLIPSTIC with single-cell transcriptomics, we build a catalogue of the immune populations that physically interact with intestinal epithelial cells at the steady state and profile the evolution of the interactome of lymphocytic choriomeningitis virus-specific CD8+ T cells in multiple organs following systemic infection. Thus, uLIPSTIC provides a broadly useful technology for measuring and understanding cell–cell interactions across multiple biological systems.

DOI: 10.1038/s41586-024-07134-4

Source: https://www.nature.com/articles/s41586-024-07134-4

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
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