小柯机器人

美国德州大学西南医学中心Daisuke Hattori研究组发现，一个快速和双向的神经活动报告器揭示了果蝇社会行为的神经关联。该研究于2023年6月12日发表于国际一流学术期刊《自然—神经科学》杂志上。

以果蝇为模型，他们开发了一种快速的双向报告器，可以提供最近神经活动的细胞读数。本报告器使用了CREB调控的转录共激活因子(CRTC)的细胞核与细胞质分布。GFP标记的CRTC (CRTC::GFP)的亚细胞分布以分钟为单位双向变化，反映了神经活动的增加和减少。他们建立了一个自动的基于机器学习的程序来有效地量化报告信号。利用这个报告器，他们证明了交配诱发的调节神经元的激活和失活。他们进一步研究了主求爱调节基因无果性(fru)的功能作用，并表明fru是确保雄性唤醒神经元被雌性信号激活的必要条件。

总之，他们的研究结果建立了CRTC::GFP作为近期神经活动的双向报告器，适用于检查行为背景下的神经相关。

据悉，神经活动在从亚秒到小时的不同时间尺度上进行调节，反映外部环境、内部状态和行为的变化。

附：英文原文

Title: A rapid and bidirectional reporter of neural activity reveals neural correlates of social behaviors in Drosophila

Author: Bonheur, Moise, Swartz, Kurtis J., Metcalf, Melissa G., Wen, Xinke, Zhukovskaya, Anna, Mehta, Avirut, Connors, Kristin E., Barasch, Julia G., Jamieson, Andrew R., Martin, Kelsey C., Axel, Richard, Hattori, Daisuke

Issue&Volume: 2023-06-12

Abstract: Neural activity is modulated over different timescales encompassing subseconds to hours, reflecting changes in external environment, internal state and behavior. Using Drosophila as a model, we developed a rapid and bidirectional reporter that provides a cellular readout of recent neural activity. This reporter uses nuclear versus cytoplasmic distribution of CREB-regulated transcriptional co-activator (CRTC). Subcellular distribution of GFP-tagged CRTC (CRTC::GFP) bidirectionally changes on the order of minutes and reflects both increases and decreases in neural activity. We established an automated machine-learning-based routine for efficient quantification of reporter signal. Using this reporter, we demonstrate mating-evoked activation and inactivation of modulatory neurons. We further investigated the functional role of the master courtship regulator gene fruitless (fru) and show that fru is necessary to ensure activation of male arousal neurons by female cues. Together, our results establish CRTC::GFP as a bidirectional reporter of recent neural activity suitable for examining neural correlates in behavioral contexts. Bonheur et al. developed a subcellular-distribution-based bidirectional neural activity reporter that operates on the timescale of minutes, and they demonstrate increases and decreases of neural activity associated with social behaviors in Drosophila.

DOI: 10.1038/s41593-023-01357-w

Source: https://www.nature.com/articles/s41593-023-01357-w

Nature Neuroscience：《自然—神经科学》，创刊于1998年。隶属于施普林格·自然出版集团，最新IF：28.771
官方网址：https://www.nature.com/neuro/
投稿链接：https://mts-nn.nature.com/cgi-bin/main.plex