小柯机器人

美国元素生物科学公司Michael Previte研究团队报道，通过Avidity测序可以在低试剂消耗的情况下实现高精度测序。这一研究成果于2023年5月25日发表在国际顶尖学术期刊《自然—生物技术》上。

他们提出了Avidity测序方法，分别优化沿着DNA模板的步骤和模板内识别每个核苷酸的过程。核苷酸鉴定使用染料标记核心上的多价核苷酸配体，形成与DNA目标克隆拷贝结合的聚合酶-聚合核苷酸复合物。这些聚合核苷酸底物，称为亲和物，降低所需的报告核苷酸的浓度，从微摩尔到纳摩尔，产生可忽略不计的解离率。

Avidity测序具有较高的准确率，在长时间的均聚物后，Avidity测序的平均错误率保持稳定。

附：英文原文

Title: Sequencing by avidity enables high accuracy with low reagent consumption

Author: Arslan, Sinan, Garcia, Francisco J., Guo, Minghao, Kellinger, Matthew W., Kruglyak, Semyon, LeVieux, Jake A., Mah, Adeline H., Wang, Haosen, Zhao, Junhua, Zhou, Chunhong, Altomare, Andrew, Bailey, John, Byrne, Matthew B., Chang, Chiting, Chen, Steve X., Cho, Byungrae, Dennler, Claudia N., Dien, Vivian T., Fuller, Derek, Kelley, Ryan, Khandan, Omid, Klein, Michael G., Kim, Michael, Lajoie, Bryan R., Lin, Bill, Liu, Yu, Lopez, Tyler, Mains, Peter T., Price, Andrew D., Robertson, Samantha R., Taylor-Weiner, Hermes, Tippana, Ramreddy, Tomaney, Austin B., Zhang, Su, Abtahi, Minna, Ambroso, Mark R., Bajari, Rosita, Bellizzi, Ava M., Benitez, Chris B., Berard, Daniel R., Berti, Lorenzo, Blease, Kelly N., Blum, Angela P., Boddicker, Andrew M., Bondar, Leo, Brown, Chris, Bui, Chris A., Calleja-Aguirre, Juan, Cappa, Kevin, Chan, Joshua, Chang, Victor W., Charov, Katherine, Chen, Xiyi, Constandse, Rodger M., Damron, Weston, Dawood, Mariam, DeBuono, Nicole, Dimalanta, John D., Edoli, Laure, Elango, Keerthana, Faustino, Nikka, Feng, Chao, Ferrari, Matthew, Frankie, Keith, Fries, Adam, Galloway, Anne, Gavrila, Vlad, Gemmen, Gregory J., Ghadiali, James, Ghorbani, Arash, Goddard, Logan A., Guetter, Adriana Roginski, Hendricks, Garren L., Hentschel, Jendrik, Honigfort, Daniel J.

Issue&Volume: 2023-05-25

Abstract: We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands on dye-labeled cores to form polymerase–polymer–nucleotide complexes bound to clonal copies of DNA targets. These polymer–nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides from micromolar to nanomolar and yield negligible dissociation rates. Avidity sequencing achieves high accuracy, with 96.2% and 85.4% of base calls having an average of one error per 1,000 and 10,000base pairs, respectively. We show that the average error rate of avidity sequencing remained stable following a long homopolymer.

DOI: 10.1038/s41587-023-01750-7

Source: https://www.nature.com/articles/s41587-023-01750-7

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex