小柯机器人

科学家利用ARPLA对单细胞中的glycoRNA进行空间成像
2023-05-28 22:26

近日,美国德克萨斯大学Yi Lu及其团队利用ARPLA对单细胞中的glycoRNA进行空间成像。相关论文于2023年5月22日在线发表在《自然—生物技术》杂志上。

研究人员报告了唾液酸适配体和RNA原位杂交介导的接近连接分析(ARPLA),以高灵敏度和选择性可视化单细胞中的糖基化RNA(glycoRNA)。ARPLA的信号输出只有在糖类和RNA的双重识别引发原位连接时才会发生,然后是互补DNA的循环放大,通过结合荧光团标记的寡核苷酸产生荧光信号。使用ARPLA,研究人员检测了细胞表面glycoRNA的空间分布和它们与脂筏的共定位,以及通过SNARE蛋白介导的分泌性胞吐进行的glycoRNA胞内运输。对乳腺细胞系的研究表明,glycoRNA与肿瘤的恶性程度和转移成反比。对glycoRNA与单核细胞-内皮细胞相互作用关系的调查表明,glycoRNA可能在免疫反应期间介导细胞-细胞相互作用。

据介绍,由于缺乏可视化方法,人们对glycoRNA的生物学作用知之甚少,这是最近发现的一类糖基化的分子。

附:英文原文

Title: Spatial imaging of glycoRNA in single cells with ARPLA

Author: Ma, Yuan, Guo, Weijie, Mou, Quanbing, Shao, Xiangli, Lyu, Mingkuan, Garcia, Valeria, Kong, Linggen, Lewis, Whitney, Ward, Carson, Yang, Zhenglin, Pan, Xingxin, Yi, S. Stephen, Lu, Yi

Issue&Volume: 2023-05-22

Abstract: Little is known about the biological roles of glycosylated RNAs (glycoRNAs), a recently discovered class of glycosylated molecules, because of a lack of visualization methods. We report sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay (ARPLA) to visualize glycoRNAs in single cells with high sensitivity and selectivity. The signal output of ARPLA occurs only when dual recognition of a glycan and an RNA triggers in situ ligation, followed by rolling circle amplification of a complementary DNA, which generates a fluorescent signal by binding fluorophore-labeled oligonucleotides. Using ARPLA, we detect spatial distributions of glycoRNAs on the cell surface and their colocalization with lipid rafts as well as the intracellular trafficking of glycoRNAs through SNARE protein-mediated secretory exocytosis. Studies in breast cell lines suggest that surface glycoRNA is inversely associated with tumor malignancy and metastasis. Investigation of the relationship between glycoRNAs and monocyte–endothelial cell interactions suggests that glycoRNAs may mediate cell–cell interactions during the immune response.

DOI: 10.1038/s41587-023-01801-z

Source: https://www.nature.com/articles/s41587-023-01801-z

Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:68.164
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex


本期文章:《自然—生物技术》:Online/在线发表

分享到:

0