小柯机器人

美国斯坦福大学Ami S. Bhatt团队近期取得重要工作进展，他们研究评估了在相对和绝对微生物组测量中样本采集引入的定量偏差。相关研究成果2023年4月27日在线发表于《自然—生物技术》杂志上。

据介绍，为了深入了解微生物测量的准确性，重要的是评估与样品条件、防腐剂方法和生物信息学分析相关的偏差来源。越来越多的证据表明，测量肠道中微生物的总数和浓度，或“绝对丰度”，比相对丰度提供了更丰富的信息来源，并可以纠正从相对丰度数据中得出的一些结论。然而，人们对防腐剂的选择如何影响这些测量结果知之甚少。

研究人员调查了两种常见的防腐剂和短期储存条件如何影响相对和绝对微生物测量。OMNIgene GUT OMR-200在不同储存温度之间产生较低的宏基因组分类变异，而Zymo DNA/RNA Shield产生较低的宏转录组分类变异。绝对丰度定量揭示了不同防腐剂中拟杆菌门和厚壁菌门比例变化的两个不同原因。

总之，基于这些结果，研究人员建议OMNIgene GUT OMR-200防腐剂用于实地研究，Zymo DNA/RNA Shield用于元转录组学研究，研究人员强烈鼓励微生物测量的绝对定量。

附：英文原文

Title: Quantifying bias introduced by sample collection in relative and absolute microbiome measurements

Author: Maghini, Dylan G., Dvorak, Mai, Dahlen, Alex, Roos, Morgan, Kuersten, Scott, Bhatt, Ami S.

Issue&Volume: 2023-04-27

Abstract: To gain insight into the accuracy of microbial measurements, it is important to evaluate sources of bias related to sample condition, preservative method and bioinformatic analyses. There is increasing evidence that measurement of the total count and concentration of microbes in the gut, or ‘absolute abundance’, provides a richer source of information than relative abundance and can correct some conclusions drawn from relative abundance data. However, little is known about how preservative choice can affect these measurements. In this study, we investigated how two common preservatives and short-term storage conditions impact relative and absolute microbial measurements. OMNIgene GUT OMR-200 yields lower metagenomic taxonomic variation between different storage temperatures, whereas Zymo DNA/RNA Shield yields lower metatranscriptomic taxonomic variation. Absolute abundance quantification reveals two different causes of variable Bacteroidetes:Firmicutes ratios across preservatives. Based on these results, we recommend OMNIgene GUT OMR-200 preservative for field studies and Zymo DNA/RNA Shield for metatranscriptomics studies, and we strongly encourage absolute quantification for microbial measurements.

DOI: 10.1038/s41587-023-01754-3

Source: https://www.nature.com/articles/s41587-023-01754-3

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex