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RIP-PEN-seq识别出一类作为剪接调节器的kink-turn RNA
2023-04-17 13:45

中山大学杨建华等研究人员合作发现,RIP-PEN-seq识别出一类作为剪接调节器的kink-turn RNA。相关论文于2023年4月10日在线发表在《自然—生物技术》杂志上。

研究人员开发了RIP-PEN-seq方法来识别被kink-turn(K-turn)结合蛋白15.5K结合的RNA的全长序列,并在人类和小鼠中发现了一类以前未被描述的具有后向K-turn模体的RNA(bktRNA)。所有bktRNA在其固定的末端位置都有两个共有序列模体,并且具有复杂的折叠特性、表达和演化模式。研究人员发现一个高度保守的bktRNA1引导甲基转移酶fibrillarin在人类中添加U12小核RNA的RNA甲基化。bktRNA1的耗竭通过损害ZCRB1对小剪接体的招募而导致U12型内含子的全局性剪接失调。大多数bktRNA通过与15.5K蛋白的相互作用调节局部内含子的剪接。

综上所述,这些研究结果描述了一类小RNA的特征,并揭示了另一层基因表达调控,涉及bktRNA、RNA剪接和RNA甲基化之间的交流。

附:英文原文

Title: RIP-PEN-seq identifies a class of kink-turn RNAs as splicing regulators

Author: Li, Bin, Liu, Shurong, Zheng, Wujian, Liu, Anrui, Yu, Peng, Wu, Di, Zhou, Jie, Zhang, Ping, Liu, Chang, Lin, Qiao, Ye, Jiayi, He, Simeng, Huang, Qiaojuan, Zhou, Hui, Chen, Jianjun, Qu, Lianghu, Yang, Jianhua

Issue&Volume: 2023-04-10

Abstract: A kink-turn (K-turn) is a three-dimensional RNA structure that exists in all three primary phylogenetic domains. In this study, we developed the RIP-PEN-seq method to identify the full-length sequences of RNAs bound by the K-turn binding protein 15.5K and discovered a previously uncharacterized class of RNAs with backward K-turn motifs (bktRNAs) in humans and mice. All bktRNAs share two consensus sequence motifs at their fixed terminal position and have complex folding properties, expression and evolution patterns. We found that a highly conserved bktRNA1 guides the methyltransferase fibrillarin to install RNA methylation of U12 small nuclear RNA in humans. Depletion of bktRNA1 causes global splicing dysregulation of U12-type introns by impairing the recruitment of ZCRB1 to the minor spliceosome. Most bktRNAs regulate the splicing of local introns by interacting with the 15.5K protein. Taken together, our findings characterize a class of small RNAs and uncover another layer of gene expression regulation that involves crosstalk among bktRNAs, RNA splicing and RNA methylation.

DOI: 10.1038/s41587-023-01749-0

Source: https://www.nature.com/articles/s41587-023-01749-0

Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:68.164
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex


本期文章:《自然—生物技术》:Online/在线发表

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