小柯机器人

美国宾夕法尼亚大学Matthew D. Weitzman团队发现，一种病毒生物分子凝聚物协调后代颗粒的组装。该研究于2023年4月5日在线发表于国际一流学术期刊《自然》。

研究人员表明，人类腺病毒52-kDa蛋白的相分离在感染性原代颗粒的协调组装中具有关键作用。我们证明，52-kDa蛋白对病毒结构蛋白组织成生物分子凝聚物至关重要。这种组织可以调节病毒的组装，使衣壳的组装与产生完整包装颗粒所需的病毒基因组的提供相协调。研究人员表明，这一功能受52-kDa蛋白的一个内在无序区域的分子语法支配，如果不能形成凝聚物或不能招募对组装至关重要的病毒因子，就会导致包装失败和只组装出非感染性颗粒。这些研究结果确定了后代颗粒协调组装的基本要求，并证明在腺病毒感染期间，病毒蛋白的相分离对生产有传染性的后代至关重要。

据了解，通过相分离形成的生物大分子凝聚物可以对细胞过程进行分隔和调节。新的证据表明，病毒感染的细胞中的无膜亚细胞区间是通过相分离形成的。虽然与几个病毒过程有关，但缺乏证据表明相分离对感染细胞中后代颗粒的组装有功能上的贡献。

附：英文原文

Title: A viral biomolecular condensate coordinates assembly of progeny particles

Author: Charman, Matthew, Grams, Nicholas, Kumar, Namrata, Halko, Edwin, Dybas, Joseph M., Abbott, Amber, Lum, Krystal K., Blumenthal, Daniel, Tsopurashvili, Elene, Weitzman, Matthew D.

Issue&Volume: 2023-04-05

Abstract: Biomolecular condensates formed by phase separation can compartmentalize and regulate cellular processes1,2. Emerging evidence has suggested that membraneless subcellular compartments in virus-infected cells form by phase separation3,4,5,6,7,8. Although linked to several viral processes3,4,5,9,10, evidence that phase separation contributes functionally to the assembly of progeny particles in infected cells is lacking. Here we show that phase separation of the human adenovirus 52-kDa protein has a critical role in the coordinated assembly of infectious progeny particles. We demonstrate that the 52-kDa protein is essential for the organization of viral structural proteins into biomolecular condensates. This organization regulates viral assembly such that capsid assembly is coordinated with the provision of viral genomes needed to produce complete packaged particles. We show that this function is governed by the molecular grammar of an intrinsically disordered region of the 52-kDa protein, and that failure to form condensates or to recruit viral factors that are critical for assembly results in failed packaging and assembly of only non-infectious particles. Our findings identify essential requirements for coordinated assembly of progeny particles and demonstrate that phase separation of a viral protein is critical for production of infectious progeny during adenovirus infection.

DOI: 10.1038/s41586-023-05887-y

Source: https://www.nature.com/articles/s41586-023-05887-y

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
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