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研究揭示人类转录-输出复合体对mRNA的识别和包装
2023-04-10 14:03

奥地利维也纳生物中心Clemens Plaschka团队揭示人类转录-输出复合体对mRNA的识别和包装。这一研究成果于2023年4月5日在线发表在国际学术期刊《自然》上。

研究人员表示,新制造的mRNA被加工和包装成成熟的核糖核蛋白复合物(mRNP),并被必需的转录-输出复合物(TREX)识别以进行核输出。然而,人们对mRNP识别和三维mRNP组织的机制了解甚少。

研究人员报告了重组的和内源性的人类mRNP与2-MDa TREX复合物结合的冷冻电镜和断层扫描结构。结果表明,mRNP是通过TREX亚单位ALYREF和mRNP结合的外显子连接复合物之间的多价相互作用来识别的。外显子连接复合物可以通过ALYREF进行多聚化,这表明了mRNP的组织机制。内源性mRNP形成紧凑的球体,被多个TREX复合物包裹。这些结果揭示了TREX如何同时识别、压实和保护mRNA,从而促进其包装来进行核输出。mRNP球的组织提供了一个框架,可用于了解mRNP结构如何促进mRNA的生物生成和输出。

附:英文原文

Title: mRNA recognition and packaging by the human transcription–export complex

Author: Pacheco-Fiallos, Beln, Vorlnder, Matthias K., Riabov-Bassat, Daria, Fin, Laura, OReilly, Francis J., Ayala, Farja I., Schellhaas, Ulla, Rappsilber, Juri, Plaschka, Clemens

Issue&Volume: 2023-04-05

Abstract: Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription–export complex (TREX) for nuclear export1,2. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood3. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export.

DOI: 10.1038/s41586-023-05904-0

Source: https://www.nature.com/articles/s41586-023-05904-0

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:69.504
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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