小柯机器人

加拿大麦吉尔大学Logan A. Walsh等研究人员合作绘制出原发性和转移性脑肿瘤的单细胞空间免疫图谱。2023年2月1日，《自然》杂志在线发表了这项成果。

研究人员应用成像细胞术来描述了139例高级别胶质瘤和46例脑转移瘤患者的免疫图谱。通过对389张高维组织病理学图像中超过110万个细胞的单细胞分析，研究人员实现了免疫谱系和激活状态的空间分辨率，并揭示了原发性肿瘤和不同实体瘤脑转移之间免疫图谱的差异。这些分析揭示了与胶质母细胞瘤患者生存相关的细胞邻近，研究人员利用这些细胞邻近鉴定了与长期生存相关的独特的髓过氧化物酶（MPO）阳性巨噬细胞群。这些发现为原发性和转移性脑肿瘤的生物学提供了见解，并加强了将空间分辨率集成到单细胞数据集来解剖肿瘤微环境背景的价值。

研究人员表示，单细胞技术使肿瘤微环境的表征达到了前所未有的深度，并揭示了肿瘤细胞及其微环境之间的巨大细胞多样性。抗肿瘤免疫依赖于肿瘤微环境中的细胞-细胞关系，然而许多单细胞研究缺乏空间背景，依赖于分离出的组织。

附：英文原文

Title: Single-cell spatial immune landscapes of primary and metastatic brain tumours

Author: Karimi, Elham, Yu, Miranda W., Maritan, Sarah M., Perus, Lucas J. M., Rezanejad, Morteza, Sorin, Mark, Dankner, Matthew, Fallah, Parvaneh, Dor, Samuel, Zuo, Dongmei, Fiset, Benoit, Kloosterman, Daan J., Ramsay, LeeAnn, Wei, Yuhong, Lam, Stephanie, Alsajjan, Roa, Watson, Ian R., Roldan Urgoiti, Gloria, Park, Morag, Brandsma, Dieta, Senger, Donna L., Chan, Jennifer A., Akkari, Leila, Petrecca, Kevin, Guiot, Marie-Christine, Siegel, Peter M., Quail, Daniela F., Walsh, Logan A.

Issue&Volume: 2023-02-01

Abstract: Single-cell technologies have enabled the characterization of the tumour microenvironment at unprecedented depth and have revealed vast cellular diversity among tumour cells and their niche. Anti-tumour immunity relies on cell–cell relationships within the tumour microenvironment1,2, yet many single-cell studies lack spatial context and rely on dissociated tissues3. Here we applied imaging mass cytometry to characterize the immunological landscape of 139 high-grade glioma and 46 brain metastasis tumours from patients. Single-cell analysis of more than 1.1 million cells across 389 high-dimensional histopathology images enabled the spatial resolution of immune lineages and activation states, revealing differences in immune landscapes between primary tumours and brain metastases from diverse solid cancers. These analyses revealed cellular neighbourhoods associated with survival in patients with glioblastoma, which we leveraged to identify a unique population of myeloperoxidase (MPO)-positive macrophages associated with long-term survival. Our findings provide insight into the biology of primary and metastatic brain tumours, reinforcing the value of integrating spatial resolution to single-cell datasets to dissect the microenvironmental contexture of cancer.

DOI: 10.1038/s41586-022-05680-3

Source: https://www.nature.com/articles/s41586-022-05680-3

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
投稿链接：http://www.nature.com/authors/submit_manuscript.html