小柯机器人

近日，德国欧洲分子生物学实验室Jan Ellenberg等研究人员合作发现，核孔组装的定量图谱揭示两种不同的机制。相关论文于2023年1月4日在线发表在《自然》杂志上。

Title: A quantitative map of nuclear pore assembly reveals two distinct mechanisms

Author: Otsuka, Shotaro, Tempkin, Jeremy O. B., Zhang, Wanlu, Politi, Antonio Z., Rybina, Arina, Hossain, M. Julius, Kueblbeck, Moritz, Callegari, Andrea, Koch, Birgit, Morero, Natalia Rosalia, Sali, Andrej, Ellenberg, Jan

Issue&Volume: 2023-01-04

Abstract: Understanding how the nuclear pore complex (NPC) is assembled is of fundamental importance to grasp the mechanisms behind its essential function and understand its role during the evolution of eukaryotes1,2,3,4. There are at least two NPC assembly pathways—one during the exit from mitosis and one during nuclear growth in interphase—but we currently lack a quantitative map of these events. Here we use fluorescence correlation spectroscopy calibrated live imaging of endogenously fluorescently tagged nucleoporins to map the changes in the composition and stoichiometry of seven major modules of the human NPC during its assembly in single dividing cells. This systematic quantitative map reveals that the two assembly pathways have distinct molecular mechanisms, in which the order of addition of two large structural components, the central ring complex and nuclear filaments are inverted. The dynamic stoichiometry data was integrated to create a spatiotemporal model of the NPC assembly pathway and predict the structures of postmitotic NPC assembly intermediates.

DOI: 10.1038/s41586-022-05528-w

Source: https://www.nature.com/articles/s41586-022-05528-w

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
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