小柯机器人

荷兰乌得勒支大学Alexander van Oudenaarden等研究人员合作利用scChIX-seq推断出单细胞中组蛋白修饰的动态关系。该项研究成果于2023年1月2日在线发表在《自然—生物技术》杂志上。

Title: scChIX-seq infers dynamic relationships between histone modifications in single cells

Author: Yeung, Jake, Florescu, Maria, Zeller, Peter, de Barbanson, Buys Anton, Wellenstein, Max D., van Oudenaarden, Alexander

Issue&Volume: 2023-01-02

Abstract: Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression. Recent advances have enabled mapping of histone marks in single cells, but most methods are constrained to profile only one histone mark per cell. Here, we present an integrated experimental and computational framework, scChIX-seq (single-cell chromatin immunocleavage and unmixing sequencing), to map several histone marks in single cells. scChIX-seq multiplexes two histone marks together in single cells, then computationally deconvolves the signal using training data from respective histone mark profiles. This framework learns the cell-type-specific correlation structure between histone marks, and therefore does not require a priori assumptions of their genomic distributions. Using scChIX-seq, we demonstrate multimodal analysis of histone marks in single cells across a range of mark combinations. Modeling dynamics of in vitro macrophage differentiation enables integrated analysis of chromatin velocity. Overall, scChIX-seq unlocks systematic interrogation of the interplay between histone modifications in single cells.

DOI: 10.1038/s41587-022-01560-3

Source: https://www.nature.com/articles/s41587-022-01560-3

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex