小柯机器人

美国纽约基因组中心Ivan Raimondi团队发现，以纳米抗体为纽带的转位法可在单细胞分辨率下进行多因素染色质分析。这一研究成果于2022年12月19日在线发表在国际学术期刊《自然—生物技术》上。

Title: Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution

Author: Stuart, Tim, Hao, Stephanie, Zhang, Bingjie, Mekerishvili, Levan, Landau, Dan A., Maniatis, Silas, Satija, Rahul, Raimondi, Ivan

Issue&Volume: 2022-12-19

Abstract: Chromatin states are functionally defined by a complex combination of histone modifications, transcription factor binding, DNA accessibility and other factors. Current methods for defining chromatin states cannot measure more than one aspect in a single experiment at single-cell resolution. Here we introduce nanobody-tethered transposition followed by sequencing (NTT-seq), an assay capable of measuring the genome-wide presence of up to three histone modifications and protein–DNA binding sites at single-cell resolution. NTT-seq uses recombinant Tn5 transposase fused to a set of secondary nanobodies (nb). Each nb–Tn5 fusion protein specifically binds to different immunoglobulin-G antibodies, enabling a mixture of primary antibodies binding different epitopes to be used in a single experiment. We apply bulk-cell and single-cell NTT-seq to generate high-resolution multimodal maps of chromatin states in cell culture and in human immune cells. We also extend NTT-seq to enable simultaneous profiling of cell surface protein expression and multimodal chromatin states to study cells of the immune system.

DOI: 10.1038/s41587-022-01588-5

Source: https://www.nature.com/articles/s41587-022-01588-5

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex