小柯机器人

北京大学Jing Wang和Chengqi Yi共同合作近期取得重要工作进展，他们研究利用GLORI对哺乳动物转录组中的单碱基m⁶A甲基化进行绝对定量分析。相关论文2022年10月27日在线发表于《自然—生物技术》杂志上。

研究人员以乙二醛和亚硝酸盐介导的未甲基化腺苷脱氨作用（GLORI）来开发一种绝对的m⁶A定量方法，该方法在概念上类似于基于亚硫酸盐测序的DNA 5-甲基胞嘧啶的定量。研究人员应用GLORI来量化小鼠和人类细胞的m⁶A甲基组，并揭示了具有不同分布和随机性的m⁶A簇状修饰。

此外，研究人员描述了m⁶A在压力下的动态，并研究了m⁶A修饰在基因表达调控中的定量情况。研究结果表明GLORI是一种无偏见且方便的m⁶A甲基组绝对定量的方法。

据介绍，N⁶-甲基腺苷（m⁶A）是哺乳动物细胞中最丰富的RNA修饰，也是研究得相对完善的表观转录组标记。尽管目前已经开发了各种绘制m⁶A图谱的工具，但目前还缺乏一种能够以单碱基分辨率绝对量化m⁶A全转录组的方法。

附：英文原文

Title: Absolute quantification of single-base m6A methylation in the mammalian transcriptome using GLORI

Author: Liu, Cong, Sun, Hanxiao, Yi, Yunpeng, Shen, Weiguo, Li, Kai, Xiao, Ye, Li, Fei, Li, Yuchen, Hou, Yongkang, Lu, Bo, Liu, Wenqing, Meng, Haowei, Peng, Jinying, Yi, Chengqi, Wang, Jing

Issue&Volume: 2022-10-27

Abstract: N6-methyladenosine (m6A) is the most abundant RNA modification in mammalian cells and the best-studied epitranscriptomic mark. Despite the development of various tools to map m6A, a transcriptome-wide method that enables absolute quantification of m6A at single-base resolution is lacking. Here we use glyoxal and nitrite-mediated deamination of unmethylated adenosines (GLORI) to develop an absolute m6A quantification method that is conceptually similar to bisulfite-sequencing-based quantification of DNA 5-methylcytosine. We apply GLORI to quantify the m6A methylomes of mouse and human cells and reveal clustered m6A modifications with differential distribution and stoichiometry. In addition, we characterize m6A dynamics under stress and examine the quantitative landscape of m6A modification in gene expression regulation. GLORI is an unbiased, convenient method for the absolute quantification of the m6A methylome.

DOI: 10.1038/s41587-022-01487-9

Source: https://www.nature.com/articles/s41587-022-01487-9

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex