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研究揭示植物中TIR1/AFB生长素受体的腺苷酸环化酶活性
2022-10-30 19:44

奥地利科学技术学院Ji Friml研究小组阐明植物中TIR1/AFB生长素受体的腺苷酸环化酶活性。相关论文于2022年10月26日在线发表在《自然》杂志上。

研究人员表示,植物激素生长素是植物发育中的主要协调信号,通过一个完善的经典信号途径介导转录重编程TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB)生长素受体是泛素连接酶复合体的F-BOX亚单位。在对生长素的反应中,它们与Aux/IAA转录抑制器相关联,并通过泛素化将它们作为降解的靶标。

研究人员发现腺苷酸环化酶(AC)活性是陆生植物中TIR1/AFB受体的一个额外功能。生长素与Aux/IAA共同刺激cAMP的产生。TIR1 C端区域AC模体的三个独立突变都废除了AC活性,每个突变都使TIR1不能有效地介导引力和持续的生长素诱导的根生长抑制,也影响生长素诱导的转录调节。这些结果突出了TIR1/AFB的交流活性在常规生长素信号中的重要性。这些数据还确定了一个独特的植物激素受体盒,其能够结合F-box和AC模体,以及cAMP作为植物第二信使的作用。

附:英文原文

Title: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants

Author: Qi, Linlin, Kwiatkowski, Mateusz, Chen, Huihuang, Hoermayer, Lukas, Sinclair, Scott, Zou, Minxia, del Genio, Charo I., Kube, Martin F., Napier, Richard, Jaworski, Krzysztof, Friml, Ji

Issue&Volume: 2022-10-26

Abstract: The phytohormone auxin is the major coordinative signal in plant development1, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.

DOI: 10.1038/s41586-022-05369-7

Source: https://www.nature.com/articles/s41586-022-05369-7

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:69.504
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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