小柯机器人

使用小鼠发育单细胞图谱系统识别细胞命运调控程序
2022-07-14 14:29

浙江大学医学院Guoji Guo和Xiaoping Han合作取得重要工作进展,他们研究利用小鼠发育单细胞图谱系统鉴定细胞命运调控程序。相关研究论文2022年7月11日在线发表于《自然—遗传学》杂志上。

研究人员在单细胞水平的发育过程中构建了小鼠谱系分化的细胞图谱,并描述了细胞类型成熟期间的谱系常见和谱系特异性调节程序。他们还发现了在无脊椎动物和脊椎动物发育过程中广泛活跃的谱系共同调控程序。特别是,研究人员将Xbp1鉴定为跨不同物种的细胞命运决定的进化保守调节因子。他们证明了Xbp1转录调控对于稳定各种小鼠细胞类型的基因调控网络很重要。他们的结果提供了对细胞基因调控程序的遗传和分子见解,并将作为进一步推进对细胞命运决定理解的基础。

据了解,沃丁顿的表观遗传景观是一个经常用来说明细胞分化的隐喻。单细胞基因组学的最新进展正在改变他们对沃丁顿景观的理解,然而细胞命运决定的分子机制仍然知之甚少。

附:英文原文

Title: Systematic identification of cell-fate regulatory programs using a single-cell atlas of mouse development

Author: Fei, Lijiang, Chen, Haide, Ma, Lifeng, E, Weigao, Wang, Renying, Fang, Xing, Zhou, Ziming, Sun, Huiyu, Wang, Jingjing, Jiang, Mengmeng, Wang, Xinru, Yu, Chengxuan, Mei, Yuqing, Jia, Danmei, Zhang, Tingyue, Han, Xiaoping, Guo, Guoji

Issue&Volume: 2022-07-11

Abstract: Waddington’s epigenetic landscape is a metaphor frequently used to illustrate cell differentiation. Recent advances in single-cell genomics are altering our understanding of the Waddington landscape, yet the molecular mechanisms of cell-fate decisions remain poorly understood. We constructed a cell landscape of mouse lineage differentiation during development at the single-cell level and described both lineage-common and lineage-specific regulatory programs during cell-type maturation. We also found lineage-common regulatory programs that are broadly active during the development of invertebrates and vertebrates. In particular, we identified Xbp1 as an evolutionarily conserved regulator of cell-fate determinations across different species. We demonstrated that Xbp1 transcriptional regulation is important for the stabilization of the gene-regulatory networks for a wide range of mouse cell types. Our results offer genetic and molecular insights into cellular gene-regulatory programs and will serve as a basis for further advancing the understanding of cell-fate decisions.

DOI: 10.1038/s41588-022-01118-8

Source: https://www.nature.com/articles/s41588-022-01118-8

Nature Genetics:《自然—遗传学》,创刊于1992年。隶属于施普林格·自然出版集团,最新IF:41.307
官方网址:https://www.nature.com/ng/
投稿链接:https://mts-ng.nature.com/cgi-bin/main.plex


本期文章:《自然—遗传学》:Online/在线发表

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