小柯机器人

英国剑桥大学Thorsten E. Boroviak课题组完成早期灵长类动物子宫内原肠运动的空间剖析。相关论文于2022年6月16日在线发表在《自然》杂志上。

研究人员通过空间转录组学和基于干细胞的胚胎模型来阐明子了宫内狨猴胚胎的早期发育。基于高斯过程回归的三维转录组描述了前内脏内胚层的出现，其特点是保守的（HHEX、LEFTY2、LHX1）和灵长类特有的（POSTN、SDC4、FZD5）因子。WNT信号在空间上协调胚盘中原条的形成，并被SFRP1/2抵消来维持前域的多能性。羊膜特化通过ID1/2/3在胚盘的边界发生，从而响应BMP信号，为灵长类多能干细胞（PSC）的羊膜分化提供了一个发育的理论依据。空间特征图表明，经过激活的狨猴PSC表现出与前胚盘的最高相似性，而初始的PSC则类似于植入前的外胚层。

这个三维转录组模型揭示了灵长类动物胚胎中谱系特化的分子代码，并为解读人类发育提供了一个体内参考。

据介绍，胚胎发育控制着早期胚胎的细胞多样性和轴型的出现。在哺乳动物中，这种转变是由胚胎和胚胎外组织界面的动态信号中心协调的。阐明体内轴形成的分子框架是我们了解人类发展的基础，并推进基于干细胞的再生方法。

附：英文原文

Title: Spatial profiling of early primate gastrulation in utero

Author: Bergmann, Sophie, Penfold, Christopher A., Slatery, Erin, Siriwardena, Dylan, Drummer, Charis, Clark, Stephen, Strawbridge, Stanley E., Kishimoto, Keiko, Vickers, Alice, Tewary, Mukul, Kohler, Timo N., Hollfelder, Florian, Reik, Wolf, Sasaki, Erika, Behr, Rdiger, Boroviak, Thorsten E.

Issue&Volume: 2022-06-16

Abstract: Gastrulation controls the emergence of cellular diversity and axis patterning in the early embryo. In mammals, this transformation is orchestrated by dynamic signalling centres at the interface of embryonic and extraembryonic tissues1–3. Elucidating the molecular framework of axis formation in vivo is fundamental for our understanding of human development4–6 and to advance stem-cell-based regenerative approaches7. Here, we illuminate early gastrulation of marmoset embryos in utero by spatial transcriptomics and stem cell-based embryo models. Gaussian process regression-based 3D-transcriptomes delineate the emergence of the anterior visceral endoderm, which is hallmarked by conserved (HHEX, LEFTY2, LHX1) and primate-specific (POSTN, SDC4, FZD5) factors. WNT signalling spatially coordinates primitive streak formation in the embryonic disc and is counteracted by SFRP1/2 to sustain pluripotency in the anterior domain. Amnion specification occurs at the boundaries of the embryonic disc through ID1/2/3 in response to BMP-signalling, providing a developmental rationale for amnion differentiation of primate pluripotent stem cells (PSCs). Spatial identity mapping demonstrates that primed marmoset PSCs exhibit highest similarity to the anterior embryonic disc, while nave PSCs resemble the preimplantation epiblast. Our 3D-transcriptome models reveal the molecular code of lineage specification in the primate embryo and provide an in vivo reference to decipher human development.

DOI: 10.1038/s41586-022-04953-1

Source: https://www.nature.com/articles/s41586-022-04953-1

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
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