小柯机器人

2022年6月13日，香港科技大学瞿佳男团队在《自然—生物技术》杂志发表论文，该研究开发出实现深度组织多光子成像的新技术。

研究人员提出了一种基于模拟锁定相位检测的焦点感应和改变（ALPHA-FSS）的自适应光学三光子显微镜。ALPHA-FSS准确地测量并有效地补偿了标本引起的像差和散射，并在深度上恢复了亚细胞分辨率。带有远程聚焦的共轭自适应光学配置能够通过完整的颅骨对小鼠皮层中的精细神经元结构进行体内成像，深度可达皮层下750微米，实现了皮层中近乎无创的高分辨率显微镜。

研究人员还展示了高灵敏度的功能钙成像和通过完整颅骨的高精度激光介导的显微手术。此外，研究人员在完整的大脑内实现了皮层深处和皮层下海马的体内高分辨率成像，最大限度地提高了皮层下1.1毫米。

据悉，由于活体物质的复杂结构引起的光学畸变和光的散射，在组织深处的高分辨率光学成像具有挑战性。

附：英文原文

Title: Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping

Author: Qin, Zhongya, She, Zhentao, Chen, Congping, Wu, Wanjie, Lau, Jackie K. Y., Ip, Nancy Y., Qu, Jianan Y.

Issue&Volume: 2022-06-13

Abstract: High-resolution optical imaging deep in tissues is challenging because of optical aberrations and scattering of light caused by the complex structure of living matter. Here we present an adaptive optics three-photon microscope based on analog lock-in phase detection for focus sensing and shaping (ALPHA-FSS). ALPHA-FSS accurately measures and effectively compensates for both aberrations and scattering induced by specimens and recovers subcellular resolution at depth. A conjugate adaptive optics configuration with remote focusing enables in vivo imaging of fine neuronal structures in the mouse cortex through the intact skull up to a depth of 750μm below the pia, enabling near-non-invasive high-resolution microscopy in cortex. Functional calcium imaging with high sensitivity and high-precision laser-mediated microsurgery through the intact skull were also demonstrated. Moreover, we achieved in vivo high-resolution imaging of the deep cortex and subcortical hippocampus up to 1.1mm below the pia within the intact brain.

DOI: 10.1038/s41587-022-01343-w

Source: https://www.nature.com/articles/s41587-022-01343-w

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex