美国马萨诸塞州综合医院Jonathan C. T. Carlson、Ralph Weissleder等研究人员合作开发出对活体细胞和组织进行时空多重免疫荧光成像的新技术。相关论文于2022年6月2日在线发表于国际学术期刊《自然—生物技术》。
Title: Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes
Author: Ko, Jina, Wilkovitsch, Martin, Oh, Juhyun, Kohler, Rainer H., Bolli, Evangelia, Pittet, Mikael J., Vinegoni, Claudio, Sykes, David B., Mikula, Hannes, Weissleder, Ralph, Carlson, Jonathan C. T.
Issue&Volume: 2022-06-02
Abstract: Cells in complex organisms undergo frequent functional changes, but few methods allow comprehensive longitudinal profiling of living cells. Here we introduce scission-accelerated fluorophore exchange (SAFE), a method for multiplexed temporospatial imaging of living cells with immunofluorescence. SAFE uses a rapid bioorthogonal click chemistry to remove immunofluorescent signals from the surface of labeled cells, cycling the nanomolar-concentration reagents in seconds and enabling multiple rounds of staining of the same samples. It is non-toxic and functional in both dispersed cells and intact living tissues. We demonstrate multiparameter (n≥14), non-disruptive imaging of murine peripheral blood mononuclear and bone marrow cells to profile cellular differentiation. We also show longitudinal multiplexed imaging of bone marrow progenitor cells as they develop into neutrophils over 6days and real-time multiplexed cycling of living mouse hepatic tissues. We anticipate that SAFE will find broad utility for investigating physiologic dynamics in living systems.
DOI: 10.1038/s41587-022-01339-6
Source: https://www.nature.com/articles/s41587-022-01339-6
Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:68.164
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex
本期文章:《自然—生物技术》:Online/在线发表