小柯机器人

中山大学眼科中心眼科学国家重点实验室Sheng Liu、刘奕志、向孟清以及同济大学医学院附属上海市第四人民医院苗智超研究组，合作利用成年猕猴海马的单细胞转录组学揭示神经前体细胞群。相关论文发表在2022年5月30日出版的《自然—神经科学》杂志上。

在这项研究中，使用优化的单细胞 RNA 测序流程，他们对成年猕猴海马的 207,785 个细胞进行了分析，并确定了 34 个细胞群，包括所有主要的海马细胞类型。对其基因表达、规范轨迹和基因调控网络的分析揭示了所有关键神经源性前体细胞群的存在，包括放射状胶质样细胞 (RGL)、中间祖细胞 (IPC) 和成神经细胞的异质池。

他们将 HMGB2 鉴定为一种新的 IPC 标记。与小鼠单细胞转录组数据的比较揭示了物种之间神经发生过程的差异。他们证实神经发生在成年灵长类动物的离体神经球培养物中得到了概括，进一步支持了能够增殖和分化的神经前体细胞 (NPC) 的存在。 他们的大规模数据集为灵长类动物提供了全面的成人神经发生图谱。

据了解，成年灵长类动物中神经发生的程度仍然存在争议。

附：英文原文

Title: Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations

Author: Hao, Zhao-Zhe, Wei, Jia-Ru, Xiao, Dongchang, Liu, Ruifeng, Xu, Nana, Tang, Lei, Huang, Mengyao, Shen, Yuhui, Xing, Changsheng, Huang, Wanjing, Liu, Xialin, Xiang, Mengqing, Liu, Yizhi, Miao, Zhichao, Liu, Sheng

Issue&Volume: 2022-05-30

Abstract: The extent to which neurogenesis occurs in adult primates remains controversial. In this study, using an optimized single-cell RNA sequencing pipeline, we profiled 207,785 cells from the adult macaque hippocampus and identified 34 cell populations comprising all major hippocampal cell types. Analysis of their gene expression, specification trajectories and gene regulatory networks revealed the presence of all key neurogenic precursor cell populations, including a heterogeneous pool of radial glia-like cells (RGLs), intermediate progenitor cells (IPCs) and neuroblasts. We identified HMGB2 as a novel IPC marker. Comparison with mouse single-cell transcriptomic data revealed differences in neurogenic processes between species. We confirmed that neurogenesis is recapitulated in ex vivo neurosphere cultures from adult primates, further supporting the existence of neural precursor cells (NPCs) that are able to proliferate and differentiate. Our large-scale dataset provides a comprehensive adult neurogenesis atlas for primates. The existence of adult neurogenesis in primates is controversial. Hao et al. performed single-cell RNA sequencing with immunostaining and neurosphere cultures on adult macaques and revealed robust neurogenesis in the adult macaque hippocampus.

DOI: 10.1038/s41593-022-01073-x

Source: https://www.nature.com/articles/s41593-022-01073-x

Nature Neuroscience：《自然—神经科学》，创刊于1998年。隶属于施普林格·自然出版集团，最新IF：28.771
官方网址：https://www.nature.com/neuro/
投稿链接：https://mts-nn.nature.com/cgi-bin/main.plex