小柯机器人

美国伊利诺伊大学厄巴纳-香槟分校Ray Ming课题组取得一项新突破。他们利用SunUp和Sunset基因组揭示了粒子轰击对木瓜转化和驯化轨迹的影响。相关论文于2022年5月12日发表在《自然-遗传学》杂志上。

研究人员在SunUp 5号染色体中鉴定了一个含三个转基因的1.64 Mb的插入，由52个核质体、21个核线粒体和1个核基因组片段组成。5号染色体中一个591.9 kb的片段被易位到该1.64 Mb插入序列。研究人员组装了Yh染色体中9.8 Mb连续的雌雄同体特异性区域和6.0 Mb的X对应区域。对86个基因组重新测序揭示了三个不同的组，验证了它们的地理起源和繁殖历史。研究发现了147次选择性进化，并确定zeta-胡萝卜素去饱和酶在驯化过程中对类胡萝卜素积累的重要作用。该研究结果阐明了粒子轰击的影响，并提高了人们对性染色体和驯化的理解，从而加快木瓜品种的改良。

研究人员表示，转基因木瓜在防范木瓜环斑病毒方面得到广泛推广。然而，粒子轰击对基因组的影响仍然未知。转基因SunUp及其祖先Sunset基因组分别占据了木瓜9条染色体的351.5和350.3Mb。

附：英文原文

Title: SunUp and Sunset genomes revealed impact of particle bombardment mediated transformation and domestication history in papaya

Author: Yue, Jingjing, VanBuren, Robert, Liu, Juan, Fang, Jingping, Zhang, Xingtan, Liao, Zhenyang, Wai, Ching Man, Xu, Xiuming, Chen, Shuai, Zhang, Shengchen, Ma, Xiaokai, Ma, Yaying, Yu, Hongying, Lin, Jing, Zhou, Ping, Huang, Yongji, Deng, Ban, Deng, Fang, Zhao, Xiaobing, Yan, Hansong, Fatima, Mahpara, Zerpa-Catanho, Dessire, Zhang, Xiaodan, Lin, Zhicong, Yang, Mei, Chen, Nancy J., Mora-Newcomer, Eric, Quesada-Rojas, Patricia, Bogantes, Antonio, Jimnez, Vctor M., Tang, Haibao, Zhang, Jisen, Wang, Ming-Li, Paull, Robert E., Yu, Qingyi, Ming, Ray

Issue&Volume: 2022-05-12

Abstract: Transgenic papaya is widely publicized for controlling papaya ringspot virus. However, the impact of particle bombardment on the genome remains unknown. The transgenic SunUp and its progenitor Sunset genomes were assembled into 351.5 and 350.3Mb in nine chromosomes, respectively. We identified a 1.64Mb insertion containing three transgenic insertions in SunUp chromosome 5, consisting of 52 nuclear-plastid, 21 nuclear-mitochondrial and 1 nuclear genomic fragments. A 591.9kb fragment in chromosome 5 was translocated into the 1.64Mb insertion. We assembled a gapless 9.8Mb hermaphrodite-specific region of the Yh chromosome and its 6.0Mb X counterpart. Resequencing 86 genomes revealed three distinct groups, validating their geographic origin and breeding history. We identified 147 selective sweeps and defined the essential role of zeta-carotene desaturase in carotenoid accumulation during domestication. Our findings elucidated the impact of particle bombardment and improved our understanding of sex chromosomes and domestication to expedite papaya improvement.

DOI: 10.1038/s41588-022-01068-1

Source: https://www.nature.com/articles/s41588-022-01068-1

Nature Genetics：《自然—遗传学》，创刊于1992年。隶属于施普林格·自然出版集团，最新IF：41.307
官方网址：https://www.nature.com/ng/
投稿链接：https://mts-ng.nature.com/cgi-bin/main.plex