小柯机器人

美国LanzaTech公司Michael Köpke等研究人员合作在工业试点规模下通过气体发酵实现丙酮和异丙醇的负碳生产。这一研究成果于2022年2月21日在线发表在国际学术期刊《自然—生物技术》上。

研究人员描述了开发一种负碳发酵路线，从丰富的、低成本的废气原料（如工业排放物和合成气）中生产工业上重要的化学品丙酮和异丙醇。利用组合途径库的方法，研究人员首先挖掘了历史上的工业菌种集合，以获得优越的酶，研究人员用这些酶来设计了自养丙酮梭菌。接下来，研究人员使用了全息分析、动力学建模和无细胞原型技术来优化流量。最后，研究人员扩大了优化菌株的规模，以高达3克/升/小时的速率和90%的选择性进行连续生产。生命周期分析证实了产品的负碳足迹。与导致释放温室气体的传统生产过程不同，这个过程可以固定碳。

这些结果表明，工程化的乙酰菌能够实现可持续的、高效率的、高选择性的化学品生产。研究人员期望这个方法可以很容易地适用于广泛的商品化学品。

据介绍，许多用化石资源生产的工业化学品可以通过发酵更可持续地生产。

附：英文原文

Title: Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale

Author: Liew, Fungmin Eric, Nogle, Robert, Abdalla, Tanus, Rasor, Blake J., Canter, Christina, Jensen, Rasmus O., Wang, Lan, Strutz, Jonathan, Chirania, Payal, De Tissera, Sashini, Mueller, Alexander P., Ruan, Zhenhua, Gao, Allan, Tran, Loan, Engle, Nancy L., Bromley, Jason C., Daniell, James, Conrado, Robert, Tschaplinski, Timothy J., Giannone, Richard J., Hettich, Robert L., Karim, Ashty S., Simpson, San D., Brown, Steven D., Leang, Ching, Jewett, Michael C., Kpke, Michael

Issue&Volume: 2022-02-21

Abstract: Many industrial chemicals that are produced from fossil resources could be manufactured more sustainably through fermentation. Here we describe the development of a carbon-negative fermentation route to producing the industrially important chemicals acetone and isopropanol from abundant, low-cost waste gas feedstocks, such as industrial emissions and syngas. Using a combinatorial pathway library approach, we first mined a historical industrial strain collection for superior enzymes that we used to engineer the autotrophic acetogen Clostridium autoethanogenum. Next, we used omics analysis, kinetic modeling and cell-free prototyping to optimize flux. Finally, we scaled-up our optimized strains for continuous production at rates of up to ~3g/L/h and ~90% selectivity. Life cycle analysis confirmed a negative carbon footprint for the products. Unlike traditional production processes, which result in release of greenhouse gases, our process fixes carbon. These results show that engineered acetogens enable sustainable, high-efficiency, high-selectivity chemicals production. We expect that our approach can be readily adapted to a wide range of commodity chemicals.

DOI: 10.1038/s41587-021-01195-w

Source: https://www.nature.com/articles/s41587-021-01195-w

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex