小柯机器人

美国斯坦福大学Stephen R. Quake研究团队近日取得一项新成果。经过不懈努力，他们研制了用无细胞RNA对妊娠期先兆子痫进行早期预测。相关论文于2022年2月9日发表于国际学术期刊《自然》杂志。

在本研究中，研究人员使用来自199名孕妇的404份血液样本来识别和验证与先兆子痫相关的循环无细胞RNA(cfRNA)转录组变化，先兆子痫是一种多器官综合征，是全球孕产妇死亡的第二大诱因。研究发现，血压正常和先兆子痫母亲之间cfRNA基因的表达在妊娠早期（即在症状出现之前）都很明显和稳定。这些变化富含特定于神经肌肉、内皮和各种类型免疫细胞和组织中的基因，这些基因反映了先兆子痫生理学的关键方面，提供了于疾病进展的新模型，并与母体器官健康相关。

该方法能够识别和独立验证一组18个基因，当在妊娠5到16周之间测量时，这些基因可以提供液体活检测试的基础，该测试将在临床症状出现之前很久就可以鉴别出有先兆子痫风险的母亲。基于这些观察结果的测试可以帮助预测和管理有先兆子痫风险的人群-这是产科护理的一个重要目标。

据了解，测量cfRNA的液体活检为以非侵入性方式研究妊娠相关并发症的发展和弥合临床护理中的空白提供了可能。

附：英文原文

Title: Early prediction of preeclampsia in pregnancy with cell-free RNA

Author: Moufarrej, Mira N., Vorperian, Sevahn K., Wong, Ronald J., Campos, Ana A., Quaintance, Cecele C., Sit, Rene V., Tan, Michelle, Detweiler, Angela M., Mekonen, Honey, Neff, Norma F., Baruch-Gravett, Courtney, Litch, James A., Druzin, Maurice L., Winn, Virginia D., Shaw, Gary M., Stevenson, David K., Quake, Stephen R.

Issue&Volume: 2022-02-09

Abstract: Liquid biopsies that measure circulating cell-free RNA (cfRNA) offer an opportunity to study the development of pregnancy-related complications in a non-invasive manner and to bridge gaps in clinical care1,2,3,4. Here we used 404 blood samples from 199 pregnant mothers to identify and validate cfRNA transcriptomic changes that are associated with preeclampsia, a multi-organ syndrome that is the second largest cause of maternal death globally5. We find that changes in cfRNA gene expression between normotensive and preeclamptic mothers are marked and stable early in gestation, well before the onset of symptoms. These changes are enriched for genes specific to neuromuscular, endothelial and immune cell types and tissues that reflect key aspects of preeclampsia physiology6,7,8,9, suggest new hypotheses for disease progression and correlate with maternal organ health. This enabled the identification and independent validation of a panel of 18 genes that when measured between 5 and 16 weeks of gestation can form the basis of a liquid biopsy test that would identify mothers at risk of preeclampsia long before clinical symptoms manifest themselves. Tests based on these observations could help predict and manage who is at risk for preeclampsia—an important objective for obstetric care10,11.

DOI: 10.1038/s41586-022-04410-z

Source: https://www.nature.com/articles/s41586-022-04410-z

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
投稿链接：http://www.nature.com/authors/submit_manuscript.html