小柯机器人

华东理工大学杨弋、陈显军等研究人员合作利用工程化的光开关RNA结合蛋白实现RNA功能和代谢的光遗传控制。该项研究成果于2022年1月3日在线发表在《自然—生物技术》杂志上。

Title: Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins

Author: Liu, Renmei, Yang, Jing, Yao, Jing, Zhao, Zhou, He, Wei, Su, Ni, Zhang, Zeyi, Zhang, Chenxia, Zhang, Zhuo, Cai, Haibo, Zhu, Linyong, Zhao, Yuzheng, Quan, Shu, Chen, Xianjun, Yang, Yi

Issue&Volume: 2022-01-03

Abstract: RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in response to blue light irradiation. When fused to various RNA effectors, LicV allows for optogenetic control of RNA localization, splicing, translation and stability in cell culture. Furthermore, LicV-assisted CRISPR–Cas systems allow for efficient and tunable photoswitchable regulation of transcription and genomic locus labeling. These data demonstrate that the photoswitchable RBP LicV can serve as a programmable scaffold for the spatiotemporal control of synthetic RNA effectors.

DOI: 10.1038/s41587-021-01112-1

Source: https://www.nature.com/articles/s41587-021-01112-1

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex