小柯机器人

近日，瑞士苏黎世联邦理工学院Grégoire Courtine、Stéphanie P. Lacour等研究人员合作建立跨越小鼠整个背腹脊髓的无线闭环光遗传学方法。2021年9月27日，国际知名学术期刊《自然—生物技术》在线发表了这一成果。

研究人员描述了一个自由活动小鼠中对整个背腹侧脊髓的目标神经元和通路进行超快速、无线、闭环操纵的系统。研究人员开发了一种柔软的可拉伸载体，集成了微型发光二极管（micro-LED），与脊髓的硬脑膜相适应。micro-LED上的硅磷基质涂层提供了机械保护和光转换，以便与大量的视蛋白兼容。

一个轻量级的头戴式无线平台为micro-LED供电，并对感应到的生理信号进行低延迟的芯片上处理，以便在一个闭环中控制光刺激。研究人员使用该设备来揭示了各种神经元亚型、感觉通路和脊柱上的投射在控制健康和脊柱受伤小鼠运动中的作用。

据介绍，光电系统可以对自由活动动物整个大脑中的目标神经元和通路进行精确控制，但用于脊髓的类似技术还没有建立起来。

附：英文原文

Title: Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice

Author: Kathe, Claudia, Michoud, Frdric, Schnle, Philipp, Rowald, Andreas, Brun, No, Ravier, Jimmy, Furfaro, Ivan, Paggi, Valentina, Kim, Kyungjin, Soloukey, Sadaf, Asboth, Leonie, Hutson, Thomas H., Jelescu, Ileana, Philippides, Antoine, Alwahab, Noaf, Gandar, Jrme, Huber, Daniel, De Zeeuw, Chris I., Barraud, Quentin, Huang, Qiuting, Lacour, Stphanie P., Courtine, Grgoire

Issue&Volume: 2021-09-27

Abstract: Optoelectronic systems can exert precise control over targeted neurons and pathways throughout the brain in untethered animals, but similar technologies for the spinal cord are not well established. In the present study, we describe a system for ultrafast, wireless, closed-loop manipulation of targeted neurons and pathways across the entire dorsoventral spinal cord in untethered mice. We developed a soft stretchable carrier, integrating microscale light-emitting diodes (micro-LEDs), that conforms to the dura mater of the spinal cord. A coating of silicone–phosphor matrix over the micro-LEDs provides mechanical protection and light conversion for compatibility with a large library of opsins. A lightweight, head-mounted, wireless platform powers the micro-LEDs and performs low-latency, on-chip processing of sensed physiological signals to control photostimulation in a closed loop. We use the device to reveal the role of various neuronal subtypes, sensory pathways and supraspinal projections in the control of locomotion in healthy and spinal-cord injured mice. Optogenetics is applied to the entire mouse spinal cord.

DOI: 10.1038/s41587-021-01019-x

Source: https://www.nature.com/articles/s41587-021-01019-x

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex