小柯机器人

研究揭示非洲爪蟾基因组的三维折叠动力学
2021-06-09 15:09

南方科技大学侯春晖等研究人员合作揭示非洲爪蟾基因组的三维折叠动力学。2021年6月7日,《自然—遗传学》杂志在线发表了这项成果。

研究人员结合高通量染色体构象捕获和基因沉默来深入了解了非洲爪蟾胚胎中的拓扑关联域(TAD)动力学。首先,非洲爪蟾中的TAD建立与小鼠和果蝇中的相似,并且不依赖于合子基因组转录激活。此过程之后是进一步活化和抑制性染色质区室的改进以及环和条纹的出现。其次,在TAD中,边界一端的较高自相互作用频率与结构蛋白CTCF和Rad21的较高DNA占据相关。第三,从头形成TAD需要染色质重塑因子ISWI。

最后,TAD结构在不同组织中是可变的。这项工作表明非洲爪蟾是染色体结构分析的强大模型,并表明染色质重塑在从头TAD建立中起着至关重要的作用。

据悉,动物间期的染色体被组织成TAD。目前尚未完全了解TAD的形成方式。

附:英文原文

Title: Three-dimensional folding dynamics of the Xenopus tropicalis genome

Author: Longjian Niu, Wei Shen, Zhaoying Shi, Yongjun Tan, Na He, Jing Wan, Jialei Sun, Yuedong Zhang, Yingzhang Huang, Wenjing Wang, Chao Fang, Jiashuo Li, Piaopiao Zheng, Edwin Cheung, Yonglong Chen, Li Li, Chunhui Hou

Issue&Volume: 2021-06-07

Abstract: Animal interphase chromosomes are organized into topologically associating domains (TADs). How TADs are formed is not fully understood. Here, we combined high-throughput chromosome conformation capture and gene silencing to obtain insights into TAD dynamics in Xenopus tropicalis embryos. First, TAD establishment in X. tropicalis is similar to that in mice and flies and does not depend on zygotic genome transcriptional activation. This process is followed by further refinements in active and repressive chromatin compartments and the appearance of loops and stripes. Second, within TADs, higher self-interaction frequencies at one end of the boundary are associated with higher DNA occupancy of the architectural proteins CTCF and Rad21. Third, the chromatin remodeling factor ISWI is required for de novo TAD formation. Finally, TAD structures are variable in different tissues. Our work shows that X. tropicalis is a powerful model for chromosome architecture analysis and suggests that chromatin remodeling plays an essential role in de novo TAD establishment. Hi-C and single-molecule sequencing analysis provide an improved assembly of the Xenopus tropicalis genome and insights into three-dimensional genome dynamics throughout embryogenesis.

DOI: 10.1038/s41588-021-00878-z

Source: https://www.nature.com/articles/s41588-021-00878-z

Nature Genetics:《自然—遗传学》,创刊于1992年。隶属于施普林格·自然出版集团,最新IF:41.307
官方网址:https://www.nature.com/ng/
投稿链接:https://mts-ng.nature.com/cgi-bin/main.plex


本期文章:《自然—遗传学》:Online/在线发表

分享到:

0