小柯机器人

美国斯坦福大学William J. Greenleaf、Georgi K. Marinov等研究人员合作在鞭毛虫中发现转录依赖的三维基因组结构。2021年4月29日，国际知名学术期刊《自然—遗传学》在线发表了这一成果。

研究人员分析了鞭毛虫Breviolum minutum的三维基因组，并发现了没有染色质环的大型拓扑结构域（鞭毛藻拓扑相关结构域，被称为“ dinoTADs”），这些环由趋同的基因阵列边界划定。转录抑制破坏了dinoTADs，这暗示转录诱导的超螺旋作用是鞭毛虫的主要拓扑力。

据介绍，鞭毛藻染色体是一种独特的进化实验，因为它们以永久性的凝结液晶状态存在；没有被组蛋白包裹； 并包含组织成串联基因阵列的基因，而转录调控却最少。

附：英文原文

Title: Transcription-dependent domain-scale three-dimensional genome organization in the dinoflagellate Breviolum minutum

Author: Georgi K. Marinov, Alexandro E. Trevino, Tingting Xiang, Anshul Kundaje, Arthur R. Grossman, William J. Greenleaf

Issue&Volume: 2021-04-29

Abstract: Dinoflagellate chromosomes represent a unique evolutionary experiment, as they exist in a permanently condensed, liquid crystalline state; are not packaged by histones; and contain genes organized into tandem gene arrays, with minimal transcriptional regulation. We analyze the three-dimensional genome of Breviolum minutum, and find large topological domains (dinoflagellate topologically associating domains, which we term ‘dinoTADs’) without chromatin loops, which are demarcated by convergent gene array boundaries. Transcriptional inhibition disrupts dinoTADs, implicating transcription-induced supercoiling as the primary topological force in dinoflagellates. Analysis of the three-dimensional architecture of the dinoflagellate Breviolum minutum genome identifies large topological domains (dinoTADs) demarcated by convergent gene array boundaries. Transcriptional inhibition disrupts dinoTADs.

DOI: 10.1038/s41588-021-00848-5

Source: https://www.nature.com/articles/s41588-021-00848-5

Nature Genetics：《自然—遗传学》，创刊于1992年。隶属于施普林格·自然出版集团，最新IF：41.307
官方网址：https://www.nature.com/ng/
投稿链接：https://mts-ng.nature.com/cgi-bin/main.plex