小柯机器人

无成像分子断层扫描实现对空间组织的分析
2021-04-22 16:16

瑞士洛桑联邦理工学院Gioele La Manno小组通过无成像分子断层扫描实现对空间组织的分析。该研究于2021年4月19日在线发表于国际一流学术期刊《自然—生物技术》。

研究人员描述了一种无需成像的框架,该框架通过将样本切成细条来实现后续的图像重建,从而在组织内定位高通量的读值。研究人员实施了此框架,从而将低输入RNA测序方案转化为无需成像的空间转录组学技术(称为STRP-seq),并通过对小鼠大脑的空间转录组进行分析来对其进行验证。

研究人员将该技术应用于了澳大利亚鬃狮蜥Pogona vitticeps的大脑。研究结果揭示了该蜥蜴端脑的分子解剖学,为爬行动物的皮层和皮下层的明显区域化提供了证据。研究人员认为,STRP-seq可用于从一系列其他组学技术中获得空间分辨的数据。

据悉,目前已经开发了几种用于空间分辨的组学分析的技术,但是每种新方法都需要设置特定的检测策略或专用仪器。

附:英文原文

Title: Spatial tissue profiling by imaging-free molecular tomography

Author: Halima Hannah Schede, Christian G. Schneider, Johanna Stergiadou, Lars E. Borm, Anurag Ranjak, Tracy M. Yamawaki, Fabrice P. A. David, Peter Lnnerberg, Maria Antonietta Tosches, Simone Codeluppi, Gioele La Manno

Issue&Volume: 2021-04-19

Abstract: Several techniques are currently being developed for spatially resolved omics profiling, but each new method requires the setup of specific detection strategies or specialized instrumentation. Here we describe an imaging-free framework to localize high-throughput readouts within a tissue by cutting the sample into thin strips in a way that allows subsequent image reconstruction. We implemented this framework to transform a low-input RNA sequencing protocol into an imaging-free spatial transcriptomics technique (called STRP-seq) and validated it by profiling the spatial transcriptome of the mouse brain. We applied the technique to the brain of the Australian bearded dragon, Pogona vitticeps. Our results reveal the molecular anatomy of the telencephalon of this lizard, providing evidence for a marked regionalization of the reptilian pallium and subpallium. We expect that STRP-seq can be used to derive spatially resolved data from a range of other omics techniques. 

DOI: 10.1038/s41587-021-00879-7

Source: https://www.nature.com/articles/s41587-021-00879-7

Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:68.164
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex


本期文章:《自然—生物技术》:Online/在线发表

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