小柯机器人

英国弗朗西斯克里克研究所Markus Ralser研究组开发出超快速蛋白质组学分析方法-Scanning SWATH。2021年3月25日出版的《自然-生物技术》杂志发表了这项成果。

他们报告了一种独立于数据的采集方法Scanning SWATH，该方法可加速质谱（MS）占空比，结合短梯度和高流量（800μl/ min-1）色谱产生定量蛋白质组。利用前驱物隔离窗口的连续移动，将前驱物质量分配给串联质谱（MS / MS）碎片痕迹，与0.5–5-min色谱梯度的传统数据独立采集（DIA）方法相比，“扫描SWATH”将前驱物鉴定提高了约70％。他们展示了超快速蛋白质组学在药物作用模式筛选和血浆蛋白质组学中的应用。扫描SWATH蛋白质组捕获了抑真菌唑类和他汀类药物的作用方式。

此外，他们确定了43个，并识别出11种新COVID-19严重程度的血浆蛋白质组学生物标志物，从而促进了患者分类和生物标志物的发现。因此，他们的结果表明，在促进蛋白质组药物筛选和临床研究的快速蛋白质组实验中，具有实质性的加速作用和增加深度。

据悉，对于大样本系列和纵向实验，蛋白质组的准确定量仍然具有挑战性。

附：英文原文

Title: Ultra-fast proteomics with Scanning SWATH

Author: Christoph B. Messner, Vadim Demichev, Nic Bloomfield, Jason S. L. Yu, Matthew White, Marco Kreidl, Anna-Sophia Egger, Anja Freiwald, Gordana Ivosev, Fras Wasim, Aleksej Zelezniak, Linda Jrgens, Norbert Suttorp, Leif Erik Sander, Florian Kurth, Kathryn S. Lilley, Michael Mlleder, Stephen Tate, Markus Ralser

Issue&Volume: 2021-03-25

Abstract: Accurate quantification of the proteome remains challenging for large sample series and longitudinal experiments. We report a data-independent acquisition method, Scanning SWATH, that accelerates mass spectrometric (MS) duty cycles, yielding quantitative proteomes in combination with short gradients and high-flow (800μlmin–1) chromatography. Exploiting a continuous movement of the precursor isolation window to assign precursor masses to tandem mass spectrometry (MS/MS) fragment traces, Scanning SWATH increases precursor identifications by ~70% compared to conventional data-independent acquisition (DIA) methods on 0.5–5-min chromatographic gradients. We demonstrate the application of ultra-fast proteomics in drug mode-of-action screening and plasma proteomics. Scanning SWATH proteomes capture the mode of action of fungistatic azoles and statins. Moreover, we confirm 43 and identify 11new plasma proteome biomarkers of COVID-19 severity, advancing patient classification and biomarker discovery. Thus, our results demonstrate a substantial acceleration and increased depth in fast proteomic experiments that facilitate proteomic drug screens and clinical studies.

DOI: 10.1038/s41587-021-00860-4

Source: https://www.nature.com/articles/s41587-021-00860-4

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex