小柯机器人

钙离子通道OSCA1.3调控植物气孔免疫
2020-08-27 15:14

英国东英吉利大学Cyril Zipfel团队在研究中取得进展。他们的研究发现钙离子(Ca2+)通道OSCA1.3调控植物气孔免疫。相关论文于2020年8月26日在线发表于国际学术期刊《自然》杂志。

研究人员发现拟南芥Ca2+渗透通道OSCA1.3调控免疫过程中的气孔关闭。一旦感知到病原体相关模式分子(PAMP),OSCA1.3就会被快速磷酸化。生化和定量磷酸化蛋白质组学分析表明,在用细菌鞭毛蛋白衍生肽PAMP flg22处理后的几分钟内,免疫受体相关胞质激酶BIK1便与OSCA1.3 N末端胞质环相互作用并使其磷酸化。

遗传和电生理数据表明,OSCA1.3是Ca2+渗透通道,而BIK1介导的其N端磷酸化可增加该通道的活性。重要的是,OSCA1.3及由BIK1介导的磷酸化对于免疫过程中气孔关闭至关重要。值得注意的是,OSCA1.3在感知到脱落酸(一种与非生物胁迫相关的植物激素)后,并未调节气孔关闭。

因此,该研究确定了植物普遍存在的Ca2+通道及其在免疫信号传递时导致气孔关闭的激活机制,并揭示了Ca2+内流机制对不同胁迫响应的特异性。

据悉,对生物和非生物胁迫的感知通常会导致植物气孔关闭。钙离子迅速跨膜进入细胞质在该反应中起着重要作用,但是该过程所涉及的Ca2+通道仍然未知。

附:英文原文

Title: The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity

Author: Kathrin Thor, Shushu Jiang, Erwan Michard, Jeoffrey George, Snke Scherzer, Shouguang Huang, Julian Dindas, Paul Derbyshire, Nuno Leito, Thomas A. DeFalco, Philipp Kster, Kerri Hunter, Sachie Kimura, Julien Gronnier, Lena Stransfeld, Yasuhiro Kadota, Christoph A. Bcherl, Myriam Charpentier, Michael Wrzaczek, Daniel MacLean, Giles E. D. Oldroyd, Frank L. H. Menke, M. Rob G. Roelfsema, Rainer Hedrich, Jos Feij, Cyril Zipfel

Issue&Volume: 2020-08-26

Abstract: Perception of biotic and abiotic stresses often leads to stomatal closure in plants1,2. Rapid influx of calcium ions (Ca2+) across the plasma membrane plays an important role in this response, but the identity of Ca2+ channels involved has remained elusive3,4. Here, we report that the Arabidopsis thaliana Ca2+-permeable channel OSCA1.3 controls stomatal closure during immunity. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phospho-proteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22 derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca2+, and that BIK1-mediated phosphorylation on its N-terminus increases this channel activity. Importantly, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immunity. Notably, OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid – a plant hormone associated with abiotic stresses. Our study thus identifies a long sought-after plant Ca2+ channel and its activation mechanisms underlying stomatal closure during immune signaling, and suggests specificity in Ca2+ influx mechanisms in response to different stresses.

DOI: 10.1038/s41586-020-2702-1

Source: https://www.nature.com/articles/s41586-020-2702-1

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:69.504
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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