小柯机器人

英国爱丁堡大学Dónal O’Carroll研究团队发现，SPOCD1是piRNA指导DNA从头甲基化的重要执行者。这一研究成果于2020年7月16日在线发表在国际学术期刊《自然》上。

Title: SPOCD1 is an essential executor of piRNA-directed de novo DNA methylation

Author: Ansgar Zoch, Tania Auchynnikava, Rebecca V. Berrens, Yuka Kabayama, Theresa Schpp, Madeleine Heep, Lina Vasiliauskait, Yuvia A. Prez-Rico, Atlanta G. Cook, Alena Shkumatava, Juri Rappsilber, Robin C. Allshire, Dnal OCarroll

Issue&Volume: 2020-07-16

Abstract: In mammals, the acquisition of the germline from the soma provides the germline with an essential challenge, the necessity to erase and reset genomic methylation1. In the male germline, RNA-directed DNA methylation silences young active transposable elements (TEs)2–4. The PIWI protein MIWI2 (PIWIL4) and its associated PIWI-interacting RNAs (piRNAs) instruct TE DNA methylation3,5. piRNAs are proposed to tether MIWI2 to nascent TE transcripts; however, the mechanism by which MIWI2 directs de novo TE methylation is poorly understood but central to the immortality of the germline. Here we define the interactome of MIWI2 in foetal gonocytes that are undergoing de novo genome methylation and identify a novel MIWI2-associated factor, SPOCD1, that is essential for young TE methylation and silencing. The loss of Spocd1 in mice results in male-specific infertility but impacts neither piRNA biogenesis nor localization of MIWI2 to the nucleus. SPOCD1 is a nuclear protein and its expression is restricted to the period of de novo genome methylation. We found SPOCD1 co-purified in vivo with DNMT3L and DNMT3A, components of the de novo methylation machinery as well as constituents of the NURD and BAF chromatin remodelling complexes. We propose a model whereby tethering of MIWI2 to a nascent TE transcript recruits repressive chromatin remodelling activities and the de novo methylation apparatus through SPOCD1. In summary, we have identified a novel and essential executor of mammalian piRNA-directed DNA methylation.

DOI: 10.1038/s41586-020-2557-5

Source: https://www.nature.com/articles/s41586-020-2557-5

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
投稿链接：http://www.nature.com/authors/submit_manuscript.html