小柯机器人

近日，比利时鲁塞尔自由大学Han Remaut及其课题组开发出新型生物纳米孔技术可高保真解析同核苷酸序列。2020年7月6日，《自然—生物技术》在线发表了这一成果。

Title: A dual-constriction biological nanopore resolves homonucleotide sequences with high fidelity

Author: Sander E. Van der Verren, Nani Van Gerven, Wim Jonckheere, Richard Hambley, Pratik Singh, John Kilgour, Michael Jordan, E. Jayne Wallace, Lakmal Jayasinghe, Han Remaut

Issue&Volume: 2020-07-06

Abstract: Single-molecule long-read DNA sequencing with biological nanopores is fast and high-throughput but suffers reduced accuracy in homonucleotide stretches. We now combine the CsgG nanopore with the 35-residue N-terminal region of its extracellular interaction partner CsgF to produce a dual-constriction pore with improved signal and base-calling accuracy for homopolymer regions. The electron cryo-microscopy structure of CsgG in complex with full-length CsgF shows that the 33 N-terminal residues of CsgF bind inside the β-barrel of the pore, forming a defined second constriction. In complexes of CsgG bound to a 35-residue CsgF constriction peptide, the second constriction is separated from the primary constriction by ~25. We find that both constrictions contribute to electrical signal modulation during single-stranded DNA translocation. DNA sequencing using a prototype CsgG–CsgF protein pore with two constrictions improved single-read accuracy by 25 to 70% in homopolymers up to 9 nucleotides long.

DOI: 10.1038/s41587-020-0570-8

Source: https://www.nature.com/articles/s41587-020-0570-8

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex