小柯机器人

德国环境卫生研究中心Pascal Falter-Braun研究组近日取得一项新成果。他们的研究揭示了通过植物激素蛋白质网络进行的广泛信号整合。这一研究成果在线发表于2020年7月1日的《自然》。

研究人员利用实验生成了拟南芥植物激素信号网络的系统图，该图由2,000多种二维蛋白质-蛋白质相互作用组成。在高度互连的网络中，研究人员确定了信号网络和数百个以前未知的信号触点，这些触点代表了潜在的串扰点。在七个激素途径中候选物功能验证揭示了84％的候选物相互作用中74％的受测蛋白质具有新功能，并且大多数信号蛋白在多种途径中均具有多效性。

此外，研究人员确定了数百种小分子依赖的激素受体相互作用。与之前的研究比较表明，非典型的和非转录介导的受体信号传导比迄今已知的更为普遍。

据介绍，植物激素通过发育过程协调对环境因素的响应，这是其抗逆能力和农艺产量的基础。遗传筛选和猜想驱动的方法已经阐明了植物激素作用的核心信号转导途径，并通过对选择途径相互作用组的研究加以扩展。但是，关于植物如何整合来自不同途径信号的基本问题仍然存在。从遗传上讲，大多数表型似乎受几种激素调控，但是转录谱分析表明激素在很大程度上引发了排它性转录进程。研究人员猜想蛋白质之间的相互作用在植物激素信号整合过程中发挥重要作用。

附：英文原文

Title: Extensive signal integration by the phytohormone protein network

Author: Melina Altmann, Stefan Altmann, Patricia A. Rodriguez, Benjamin Weller, Lena Elorduy Vergara, Julius Palme, Nora Marn-de la Rosa, Mayra Sauer, Marion Wenig, Jos Antonio Villacija-Aguilar, Jennifer Sales, Chung-Wen Lin, Ramakrishnan Pandiarajan, Veronika Young, Alexandra Strobel, Lisa Gross, Samy Carbonnel, Karl G. Kugler, Antoni Garcia-Molina, George W. Bassel, Claudia Falter, Klaus F. X. Mayer, Caroline Gutjahr, A. Corina Vlot, Erwin Grill, Pascal Falter-Braun

Issue&Volume: 2020-07-01

Abstract: Plant hormones coordinate responses to environmental cues with developmental programs1, and are fundamental for stress resilience and agronomic yield2. The core signalling pathways underlying the effects of phytohormones have been elucidated by genetic screens and hypothesis-driven approaches, and extended by interactome studies of select pathways3. However, fundamental questions remain about how information from different pathways is integrated. Genetically, most phenotypes seem to be regulated by several hormones, but transcriptional profiling suggests that hormones trigger largely exclusive transcriptional programs4. We hypothesized that protein–protein interactions have an important role in phytohormone signal integration. Here, we experimentally generated a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein–protein interactions. In the highly interconnected network, we identify pathway communities and hundreds of previously unknown pathway contacts that represent potential points of crosstalk. Functional validation of candidates in seven hormone pathways reveals new functions for 74% of tested proteins in 84% of candidate interactions, and indicates that a large majority of signalling proteins function pleiotropically in several pathways. Moreover, we identify several hundred largely small-molecule-dependent interactions of hormone receptors. Comparison with previous reports suggests that noncanonical and nontranscription-mediated receptor signalling is more common than hitherto appreciated.

DOI: 10.1038/s41586-020-2460-0

Source: https://www.nature.com/articles/s41586-020-2460-0

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
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