小柯机器人

德国马克斯·普朗克光科学研究所Vahid Sandoghdar研究组，对多分散体中弱散射纳米粒子的精确尺寸和折射率进行了分析。相关论文于2022年5月9日发表在《自然-方法学》杂志上。

研究人员研发了一种名为iNTA的新方法，它将散射的干涉检测与纳米粒子跟踪分析相结合，具有很高的灵敏度和精度，从而确定悬浮液中纳米粒子的尺寸和折射率分布。在用胶体金样品对iNTA进行基准测试后，研究人员展示了它在解析已知来源的各种多组分和多分散样品成分方面的卓越能力。

此外，研究人员通过阐明利什曼原虫和人类尿液中细胞外囊泡的折射率和大小分布展示了该方法的应用。已经利用几个重要的应用程序优化了iNTA的性能，但其仍存在改进的可能性。

据悉，对液体悬浮液中颗粒尺寸和材料特性的表征有很高要求，例如，在分析胶体样品或体液（如尿液或血浆）时。然而，现有方法在揭示样本真实成分方面受到限制。

附：英文原文

Title: Precision size and refractive index analysis of weakly scattering nanoparticles in polydispersions

Author: Kashkanova, Anna D., Blessing, Martin, Gemeinhardt, Andr, Soulat, Didier, Sandoghdar, Vahid

Issue&Volume: 2022-05-09

Abstract: Characterization of the size and material properties of particles in liquid suspensions is in very high demand, for example, in the analysis of colloidal samples or of bodily fluids such as urine or blood plasma. However, existing methods are limited in their ability to decipher the constituents of realistic samples. Here we introduce iNTA as a new method that combines interferometric detection of scattering with nanoparticle tracking analysis to reach unprecedented sensitivity and precision in determining the size and refractive index distributions of nanoparticles in suspensions. After benchmarking iNTA with samples of colloidal gold, we present its remarkable ability to resolve the constituents of various multicomponent and polydisperse samples of known origin. Furthermore, we showcase the method by elucidating the refractive index and size distributions of extracellular vesicles from Leishmania parasites and human urine. The current performance of iNTA already enables advances in several important applications, but we also discuss possible improvements. iNTA combines interferometric detection of scattering with nanoparticle tracking analysis for determining the size and refractive index distributions of nanoparticles in suspension with high sensitivity and precision.

DOI: 10.1038/s41592-022-01460-z

Source: https://www.nature.com/articles/s41592-022-01460-z

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex