小柯机器人

挪威科技大学Edvard I. Moser、宗伟健等研究人员合作实现自由活动小鼠的大规模双光子钙成像。该项研究成果于2022年3月18日在线发表在《细胞》杂志上。

研究人员开发了一种小型化的双光子显微镜（MINI2P），用于对自由移动的小鼠进行快速、高分辨率、多平面的钙成像，一次可观察1000多个神经元。显微镜重量低于3g，连接线高度灵活，与未固定、未植入的动物相比，MINI2P可以在各种实验中稳定成像，不妨碍行为。细胞产量的提高是通过光学系统的设计实现的，该系统具有更大的视野（FOV）和微调镜头，增加了Z轴扫描范围和速度，可以快速稳定地对多个交错的平面进行成像，并进行三维功能成像。跨越多个相邻FOV的连续成像使同一动物中超过10,000个神经元的记录成为可能。

研究人员从视觉皮层、内侧内嗅皮层和海马的细胞群中获得了大规模的原理验证数据，从而揭示了所有区域细胞的空间调谐。

附：英文原文

Title: Large-scale two-photon calcium imaging in freely moving mice

Author: Weijian Zong, Horst A. Obenhaus, Emilie R. Skyten, Hanna Eneqvist, Nienke L. de Jong, Ruben Vale, Marina R. Jorge, May-Britt Moser, Edvard I. Moser

Issue&Volume: 2022-03-18

Abstract: We developed a miniaturized two-photon microscope (MINI2P) for fast, high-resolution, multiplane calcium imaging of over 1,000 neurons at a time in freely moving mice. With a microscope weight below 3 g and a highly flexible connection cable, MINI2P allowed stable imaging with no impediment of behavior in a variety of assays compared to untethered, unimplanted animals. The improved cell yield was achieved through a optical system design featuring an enlarged field of view (FOV) and a microtunable lens with increased z-scanning range and speed that allows fast and stable imaging of multiple interleaved planes, as well as 3D functional imaging. Successive imaging across multiple, adjacent FOVs enabled recordings from more than 10,000 neurons in the same animal. Large-scale proof-of-principle data were obtained from cell populations in visual cortex, medial entorhinal cortex, and hippocampus, revealing spatial tuning of cells in all areas.

DOI: 10.1016/j.cell.2022.02.017

Source: https://www.cell.com/cell/fulltext/S0092-8674(22)00197-0