Table of Fluorochromes
This is a table of characteristics for fluorochromes used in flow cytometry, within groups, roughly in order of excitation wavelength. Colors you might see with a capable browser are only a rough approximation.
Probe
Ex (nm)
Em (nm)
MW
Notes
Reactive and conjugated probes
Hydroxycoumarin
325
386
331
Succinimidyl ester
Aminocoumarin
350
445
330
Succinimidyl ester
Methoxycoumarin
360
410
317
Succinimidyl ester
Cascade Blue
375;400
423
596
Hydrazide
Lucifer yellow
425
528
NBD
466
539
294
NBD-X
R-Phycoerythrin (PE)
480;565
578
240,000
PE-Cy5 conjugates
480;565;65 0
670
aka Cychrome, R670, Tri-Color, Quantum Red
PE-Cy7 conjugates
480;565;74 3
767
Red 613
480;565
613
PE-Texas Red
Fluorescein
495
519
389
FITC; pH sensitive
BODIPY-FL
503
512
Cy3
512;552
565,615
TRITC
547
572
444
TRITC
X-Rhodamine
570
576
548
XRITC
Lissamine Rhodamine B
570
590
PerCP
490
675
Peridinin chlorphyll protein
Texas Red
589
615
625
Sulfonyl chloride
Cy5
625-650
670
Cy7
743
767
1,001
Allophycocyanin (APC)
650
660
104,000
TruRed
490,675
695
PerCP-Cy5.5 conjugate
APC-Cy7 conjugates
650;755
767
PharRed
Nucleic acid probes
Hoechst 33342
343
483
616
AT-selective
DAPI
345
455
AT-selective
Hoechst 33258
345
478
624
AT-selective
SYTOX Blue
431
480
~400
DNA
Chromomycin A3
445
575
CG-selective
Mithramycin
445
575
YOYO-1
491
509
1270.65
SYTOX Green
504
523
~600
DNA
SYTOX Orange
547
570
~500
DNA
Ethidium Bromide
493
620
394.31
7-AAD
546
647
7-aminoactinomycin D, CG-selective
Acridine Orange
503
530/640
DNA/RNA
TOTO-1, TO-PRO-1
509
533
Vital stain, TOTO: Cyanine Dimer TO-PRO: Cyanine Monomer
Thiazole Orange
510
530
Propidium Iodide (PI)
536
617
668.4
TOTO-3, TO-PRO-3
642
661
LDS 751
543;590
712;607
471.98
DNA (543ex/712em), RNA (590ex/607em)
Cell function probes
Indo-1
361/330
490/405
1010
AM ester. Low/High Ca ++,
Fluo-3
506
526
855
AM ester. pH > 6
DCFH
505
535
529
2'7'Dichorodihydrofluorescein, oxidized form
DHR
505
534
346
Dihydrorhodamine 123, oxidized form, light catalyzes oxidation
SNARF
548/579
587/635
pH 6/9
Fluorescent Proteins
Y66F
360
508
Y66H
360
442
EBFP
380
440
(Clonetech ) Quantum yield 0.18
Wild Type
396,475
508,503
GFPuv
385
508
(Clonetech )
ECFP
434
477
(Clonetech ) Quantum yield 0.40
Y66W
436
485
S65A
471
504
S65C
479
507
S65L
484
510
S65T
488
511
EGFP
489
508
(Clonetech ) Quantum yield 0.60
EYFP
514
527
(Clonetech ) Quantum yield 0.61
DsRed
558
583
(Clonetech ) Quantum yield 0.29
Other probes
Monochlorobimane
380
461
226
Glutathione probe
Calcein
496
517
623
pH > 5
Legend:
? Ex: Peak excitation wavelength (nm)
? Em: Peak emission wavelength (nm)
? MW: Molecular weight
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