博文
Sutter P-97 micropipette puller 笔记
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HEAT: |
= Ramp - 5 to Ramp + 15 for most applications! |
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PULL: |
= 0 Patch, 3-5mm taper and 1-3μm tips |
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= 30 -70 Microinjection, 6-8mm tapers, 0.9 to 0.5μm tips |
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= 70 - 150 High MΩ, 9-15mm tapers, 0.5 to 0.06μm tips |
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VELOCITY: |
= 20 - 60 Patch, 3-5mm taper and 1-3μm tips |
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= 50 - 80 Microinjection, 6-8mm tapers, 0.9 to 0.5μm tips |
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= 70 - 100 High MΩ, 9-15mm tapers, 0.5 to 0.06μm tips |
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TIME: |
= 150 trough, 250 box Patch, 3-5mm taper and 1-3μm tips |
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= 150 trough, 250 box Microinjection, 6-8mm tapers, 0.9 to 0.5μm tips |
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= 150 trough, 250 box High MΩ, 9-15mm tapers, 0.5 to 0.06μm tips |
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DELAY: |
= 1 Patch, 3-5mm taper and 1-3μm tips |
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= 60 - 110 Microinjection, 6-8mm tapers, 0.9 to 0.5μm tips |
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= 40 - 90 High MΩ, 9-15mm tapers, 0.5 to 0.06μm tips |
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PRESSURE: |
= 200 - 500 Thin Walled Glass |
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= 200 - 700 Thick Walled Glass |
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PARAMETER |
INCREASE |
DECREASE |
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Heat |
Longer Taper |
Shorter Taper |
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Higher Resistance |
Lower Resistance |
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Pull |
Smaller Tips |
Larger Tips |
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Longer Taper |
Shorter Taper |
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Velocity |
Smaller Tips |
Larger Tips |
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Time |
Shorter Taper |
Longer Taper |
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(cooling time-ms) |
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Pressure |
Shorter Taper |
Longer Taper |
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(air flow-psi) |
Lower Resistance |
Higher Resistance |
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