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云师大化工学院胡蓉教授课题组在top期刊《Food Chemistry》发表最新研究成果

已有 836 次阅读 2023-11-22 14:16 |个人分类:云师大研究|系统分类:论文交流

2023年11月11日,Elsevier 旗下top期刊《Food Chemistry》在线发表了云南师范大学化学与化工学院胡蓉教授课题组与昆明理工大学真空冶金及材料研究所姚耀春教授课题组最新研究成果《Colorimetric and photothermal dual-mode aptasensor with redox cycling amplification for the detection of ochratoxin A in corn samples》。云南师范大学化学化工学院胡蓉教授和昆明理工大学真空冶金及材料研究所姚耀春教授为共同通讯作者。

https://doi.org/10.1016/j.foodchem.2023.137968

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Abstract

Ochratoxin A (OTA) detection is critical for public health safety. This study proposes a G-quadruplex-Hemin/iodide (G4-Hemin/I)-mediated non-enzyme redox cycling amplification (RCA) system for dual-modal (colorimetric and photothermal thermometer) OTA analysis. The proposed aptasensor platform for point-of-care testing employs a common thermometer for quantitative signal readouts. The OTA aptamer folds into a G4 structure, which significantly enhances the catalytic activity in the presence of I after RCA reaction. Moreover, a notable temperature enhancement causes color changes, providing an ultrasensitive and label-free platform for OTA detection. Further, the designed sensor was applied to OTA content determination in corn samples and achieved satisfactory results compared to a commercial enzyme-linked immunoassay kit. The proposed dual-mode aptasensor is simple, highly sensitive (1 pg/mL for colorimetric method, 0.8 pg/mL for photothermal method), selective, and suitable for low-cost instrument-free bioanalysis in low-resource settings.

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云南师范大学生物电分析领域中青年学者之 胡蓉




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