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云师大杨云慧课题组在《生物传感与生物电子》Biosensors & bioelectronics杂志上发表第3篇论文

已有 1368 次阅读 2022-4-17 10:46 |个人分类:云师大研究|系统分类:论文交流

1-s2.0-S0956566322002810-gr3_lrg.jpgA visual peroxidase mimicking aptasensor based on Pt nanoparticles-loaded on iron metal organic gel for fumonisin B<sub>1</sub> analysis in corn meal.

By:

Li, MeiLi, De YanLi, Zi YingHu, RongYang, Yun HuiYang, TongBiosensors & bioelectronics

Volume 209

Page 114241

DOI 10.1016/j.bios.2022.114241

Published 2022-Apr-05

Indexed 2022-04-13

Document Type 

Journal Article

Abstract

A visual colorimetric aptasensor combining platinum nanoparticles (Pt NPs) loaded on Fe-based metal organic gel (Fe-MOG) as peroxidase mimics with magnetic separation technique was designed for the detection of fumonisin B1 (FB1). Interestingly, based on the inherent properties of Fe-MOG such as porous nanostructures, abundant Fe2+/Fe3+ active metal centers and synergetic effect between Pt NPs and Fe-MOG, Pt NPs/Fe-MOG composites can be employed as excellent peroxidase mimetic enzyme to oxidize 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. This process was accompanied by a color variation of the solution from colorless to blue. Kinetic analysis showed that the Pt NPs/Fe-MOG composites make an effective peroxidase mimic with low Michaelis constant (Km), high substrate binding affinity and rapid catalytic velocity (Vm). Furthermore, the Pt NPs/Fe-MOG based colorimetric aptasensor was used to detect FB1. The prepared Pt NPs/Fe-MOG achieved lower detection limit (2.7pg/mL, 3sigma/k) with a wide linear range of 0.01-2000.0ng/mL. Finally, as a proof of concept of the rapidity and the simplicity of this colorimetric aptasensor, Pt NPs/Fe-MOG was used for the detection of FB1 in real corn samples.

Copyright © 2022 Elsevier B.V. All rights reserved.

Keywords

Keyword List

AptasensorColorimetric analysisCorn sampleFumonisin B(1) (FB(1))Metal-organic gels (MOGs)Peroxidase mimicking

Author Information

Addresses

College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China.College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China. Electronic address: yyhui2002@aliyun.com.College of Chemistry and Chemical Engineering, Yunnan Normal University, Yunnan, Kunming, 650500, PR China. Electronic address: yt09132149@163.com.

Journal information Biosensors & bioelectronics eISSN : 1873-4235

1-s2.0-S0956566322002810-sc1_lrg.jpg



1.A visual peroxidase mimicking aptasensor based on Pt nanoparticles-loaded on iron metal organic gel for fumonisin B<sub>1</sub> analysis in corn meal.

Li, MeiLi, De Yan(...); Yang, Tong2022-Apr-05 | Biosensors & bioelectronics 209 , pp.114241

A visual colorimetric aptasensor combining platinum nanoparticles (Pt NPs) loaded on Fe-based metal organic gel (Fe-MOG) as peroxidase mimics with magnetic separation technique was designed for the detection of fumonisin B1 (FB1). Interestingly, based on the inherent properties of Fe-MOG such as porous nanostructures, abundant Fe2+/Fe3+ active metal centers and synergetic effect between Pt NPs

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2.2D-porphrinic covalent organic framework-based aptasensor with enhanced photoelectrochemical response for the detection of C-reactive protein

Zhang, XChi, KN(...); Yang, YHMar 15 2019 | BIOSENSORS & BIOELECTRONICS 129 , pp.64-71

In this study, a novel photoelectrochemical (PEC) aptasensor based on two-dimensional (2D) porphyrinic covalent organic frameworks (p-COFs) for the label-free detection of C-reactive protein (CRP) is presented. The obtained p-COFs possess high conductivity and an improved stability due to strong and rigid covalent linkages. The introduction of p-COFs hinder the recombination of electrons and ho

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3. A universal aptameric biosensor: Multiplexed detection of small analytes via aggregated perylene-based broad-spectrum quencher


Hu, RZhang, X(...); Zhang, XBJun 15 2017 | BIOSENSORS & BIOELECTRONICS 92 , pp.40-46

A universal aptameric system based on the taking advantage of double-stranded DNA/perylene diimide (dsDNA/PDI) as the signal probe was developed for multiplexed detection of small molecules. Aptamers are single-stranded DNA or RNA oligonucleotides which are selected in vitro by a process known as systematic evolution of ligands by exponential enrichment. In this work, we synthesized a new kind

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