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全脑缺血可出现于心脏停止这样的紧急情况,氢气通过抗氧化作用对脑局部缺血有治疗效果,去年有欧洲学者报道呼吸停止引起的脑损伤与此有关,主要是氧气不足的问题,全脑缺血不仅是缺氧而且是缺血。全脑缺血的模型比较常用的是四动脉模型,具体就是预先把锥动脉阻断,次日进行颈总动脉阻断在恢复血流,也就是说并非是完全持续阻断,这类似与心脏停止跳动后的复苏情况。来自南京军区总医院麻醉科的研究表明,氢气生理盐水能治疗这类疾病,也就是说氢气对那些因自缢、心脏停止的复苏患者有治疗价值。研究观察了神经组织染色、细胞凋亡染色、炎症因子和氧化损伤等指标,确认这种效果,特别重要的是,即使在缺血再灌注后6小时给氢气,这种效果仍存在。这一点非常重要,因为说明即使延迟治疗仍有可能有效果。
本文将发表在外科研究杂志。Journal of Surgical Research, In Press, Uncorrected Proof, Available online 2 March 2011
The Effect of Hydrogen-Rich Saline on the Brain of Rats with Transient Ischemia.pdf
Qing Ji, Kangli Hui, Lidong Zhang, Xuejun Sun, Weiyan Li, Manlin Duan
Background
Due to its antioxidant and anti-inflammatory properties, hydrogen gas (H2) has protective effects on a variety of organs from damage induced by ischemia/reperfusion (I/R). In this study, we tested the protective effect of hydrogen-rich saline on the brain in a global cerebral I/R model.
Materials and Methods
We used a four-vessel occlusion model of global cerebral ischemia (15 min) and reperfusion, with rats. The rats were divided into four groups (n = 96: sham, I/R plus physiologic saline injected intraperitoneally, I/R plus hydrogen-rich saline injected intraperitoneally at the beginning of reperfusion, and I/R plus hydrogen-rich saline injected intraperitoneally 6 h after reperfusion began. One group of rats was sacrificed after 24 h of reperfusion. Malondialdehyde (MDA) was measured to quantify the oxidative stress. Capspase-3 was measured to indicate the status of apoptosis. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and nuclear factor-κB (NF-κB) were measured to monitor the inflammation. Another group of rats was sacrificed after 72 h of reperfusion to measure the histologic damages in hippocampus by hematoxylin and eosin staining and Nissl staining.
Results
Compared with rats with I/R only, hydrogen-rich saline treatment significantly improved the amount of surviving cells. NF-κB, TNF-α, IL-6, MDA, and caspase-3 were all increased significantly by I/R injury. Hydrogen-rich saline reduced all these markers.
Conclusions
Our data demonstrate that intraperitoneal injection of hydrogen-rich saline has strong protective effect on the transient global cerebral ischemia-reperfusion rats.
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